细胞内蛋白质磁共振

波谱学杂志 ›› 2012, Vol. 29 ›› Issue (4): 475-488.

• 特邀综述 • 下一篇

细胞内蛋白质磁共振

王亚强，李从刚，PIELAK Gary J

1. 北卡罗来纳大学教堂山分校化学系，美国教堂山 27599； 
2. 波谱与原子分子物理国家重点实验室，武汉磁共振中心(中国科学院武汉物理与数学研究所)，湖北武汉 430071； 
3. 北卡罗来纳大学教堂山分校生物化学与生物物理系，美国教堂山 27599；
4. 北卡罗来纳大学教堂山分校莱恩伯格综合癌症中心，美国教堂山 27599

收稿日期:2012-02-01 修回日期:2012-02-23 出版日期:2012-12-05 发布日期:2012-12-05
基金资助:
National Science Foundation Grant (MCB-1051819) and National Science Foundation of China (21075134).

In-Cell Protein Magnetic Resonance Spectroscopy

WANG Ya-Qiang, LI Cong-Hang, PIELAK Gary J

1. Department of Chemistry, University of North Carolina, Chapel Hill 27599, USA;
2. State Key Laboratory of Magnetic Resonance and Molecular and Atomic Physics, Wuhan Center for Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
3. Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599, USA;
4. Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599, USA

Received:2012-02-01 Revised:2012-02-23 Online:2012-12-05 Published:2012-12-05
Supported by:
National Science Foundation Grant (MCB-1051819) and National Science Foundation of China (21075134).

摘要/Abstract

摘要：

细胞内的环境异常复杂，其中生物大分子的浓度超过300 mg/mL并占据着大约30%的细胞内空间. 然而，直到目前为止大多数的蛋白质研究仍然在细胞外或是甚至非常稀的缓冲液中进行. 细胞内磁共振（In-cell NMR）提供了一个可以直接非损伤地获取细胞内原子水平信息的方法. 虽然In-cell NMR在近10年里有了较大的发展，但是这一技术尚未成熟. 在该篇综述中，作者首先总结制约这一技术发展的因素以及研究中需要注意的事项，最后讨论了未来的发展方向.

关键词: 细胞内磁共振(In-cell NMR)；蛋白质；大肠杆菌；顺磁共振(EPR)

Abstract:

The cellular interior contains macromolecules at concentrations exceeding 300 mg/mL and volume occupancies of 30%. Until recently, however, most protein studies were performed in vitro and in dilute solutions. In-cell NMR spectroscopy provides a means to gain atomiclevel data noninvasively in the cellular environment. Although in-cell NMR has recently undergone significant advances, it is still in its infancy. Here, we review the limitations and potential pitfalls of in-cell NMR and recent advances in the field.

Key words: in-cell NMR, protein, Escherichia coli, EPR

中图分类号:

O482.53

王亚强, 李从刚, PIELAK Gary J. 细胞内蛋白质磁共振[J]. 波谱学杂志, 2012, 29(4): 475-488.

WANG Ya-Qiang, LI Cong-Hang, PIELAK Gary J. In-Cell Protein Magnetic Resonance Spectroscopy[J]. Chinese Journal of Magnetic Resonance, 2012, 29(4): 475-488.

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细胞内蛋白质磁共振

In-Cell Protein Magnetic Resonance Spectroscopy

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