EPR Studies on Acetyl-CoA Synthase/Carbon Monoxide Dehydrogenase

Abstract

Abstract:

Actyl-CoA synthase/carbon monoxide dehydogenase (ACS/CODH) from anaerobic microbes is a Ni, Fe-containing metalloenzyme, which contains up to seven metal clusters. It catalyses the reversible conversion of CO₂ and CO, and utilizes CO and/or CO as carbon source and H₂as energy to synthesize actyl-CoA. Interests in these enzymes have been increased in recent years due to energy crisis and environmental issues such as greenhouse effects. As one of the most effective techniques to detect the valence state and coordination of metals, EPR has been used to study the enzyme since 1980s. In this paper, the structures of metal clusters, function and catalytic mechanism of ACS/CODH studied by EPR were summarized and reviewed.

Key words: EPR, , actyl-CoA synthase/carbon monoxide dehydogenase, metalloenzyme, metalloprotein, enzymology

CLC Number:

O482.53

LIU Yi, TAN Xiang-Shi. EPR Studies on Acetyl-CoA Synthase/Carbon Monoxide Dehydrogenase[J]. Chinese Journal of Magnetic Resonance, 2011, 28(4): 490-501.

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