基于1H NMR 技术的锥叶柴胡与北柴胡鉴别研究

波谱学杂志

基于¹H NMR 技术的锥叶柴胡与北柴胡鉴别研究

宋爱华1，沙沂1，徐晓雪2，李文1，许枬2*，李晓鹏2，韩伟健2，林晓彤2

1.沈阳药科大学测试中心，辽宁沈阳 110016；2. 辽宁中医药大学药学院，辽宁大连 116600

收稿日期:2013-07-08 修回日期:2013-07-26 出版日期:2014-06-05 发布日期:2014-06-05
作者简介:宋爱华(1959-)，女，副教授，研究方向为天然化学成分核磁共振谱学特征研究，电话：024 -23986332；E-mail: shayihc@163.com. *通讯联系人：许枬，电话：0411-87586014；E-mail: xudanbs@163.com.
基金资助:
国家公益性行业专项课题资助项目(201007007).

Differentiating Bupleurum bicaule from Bupleurum chinense Using ¹H NMR Spectroscopy

SONG Ai-hua¹，SHA Yi¹，XU Xiao-xue²，LI Wen¹，XU Nan^2*，LI Xiao-peng²，HAN Wei-jian²，LIN Xiao-tong²

1. Testing center, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China

Received:2013-07-08 Revised:2013-07-26 Online:2014-06-05 Published:2014-06-05
About author:*Corresponding author：XU Nan, Tel: 0411-87586014; E-mail: xudanbs@163.com.
Supported by:
国家公益性行业专项课题资助项目(201007007).

摘要/Abstract

摘要：

柴胡是疏肝理气的中药．我国药典规定柴胡的植物来源为伞形科植物柴胡Bupleurum chinense DC. 或狭叶柴胡 Bupleurum scorzoneri folium Willd.的干燥根，习称“北柴胡”和“南柴胡”．但由于地区用药习惯和品种混淆，在实际应用和商品流通中多种柴胡属植物的根均作为柴胡入药．锥叶柴胡由于习惯用名与正品北柴胡混淆，常做北柴胡使用，但尚无健全的鉴别二者的方法．该研究运用基于¹H NMR技术的代谢组学方法研究了锥叶柴胡与北柴胡的化学成分，结果表明锥叶柴胡中柴胡皂苷元E、F、G和前柴胡皂苷F、G、A含量较高，而北柴胡中柴胡皂苷a、b、c含量较高．采用PCA和PLS-DA分析锥叶柴胡和北柴胡¹H NMR谱图，结果显示二者差异明显，δ 0.84、0.88、1.24、1.28、1.32、1.36、2.12、4.40、4.32、4.56处的特征信号可以用于准确鉴别锥叶柴胡和北柴胡．该文所建立的北柴胡及其混品判别模式可以准确的鉴别锥叶柴胡和北柴胡，为柴胡的临床鉴别使用提供依据．

关键词: 核磁共振氢谱(¹H NMR), 柴胡鉴别, 多元分析, 二次代谢产物

Abstract:

Bupleuuri Radix(Chaihu in Chinese)is a widely utilized traditional Chinese medicine for soothing liver and regulating vital energy. According to Chinese pharmacopoeias,only the roots of Bupleurum chinense are considered to be Bei Chaihu. In practice, However, roots of other Bupleurum plants, such as Bupleurum bicaule, are also sold or used as Bei Chaihu. In order to find to analytical tool to differentiate the roots of Bupleurum chinense and Bupleurum bicaule, we used ¹H NMR to analyze the composition of secondary metabolites of the two species. It was found that the metabolic fingerprint of the Bupleurum chinense is different from that of the Bupleurum bicaule, and thus ¹H NMR can be used to differentiate the two.

Key words: ¹H NMR, Bupleuri Radix, PLS-DA, secondary metabolites

中图分类号:

O482.53

宋爱华1，沙沂1，徐晓雪2，李文1，许枬2*，李晓鹏2，韩伟健2，林晓彤2. 基于¹H NMR 技术的锥叶柴胡与北柴胡鉴别研究[J]. 波谱学杂志.

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基于¹H NMR 技术的锥叶柴胡与北柴胡鉴别研究

Differentiating Bupleurum bicaule from Bupleurum chinense Using ¹H NMR Spectroscopy

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