颐脑解郁方对抑郁大鼠脑1H波谱的干预作用

波谱学杂志 ›› 2012, Vol. 29 ›› Issue (3): 379-387.

颐脑解郁方对抑郁大鼠脑¹H波谱的干预作用

黄育玲¹，唐启盛^1*，李宁²，朱晨军¹

1. 北京中医药大学第三附属医院，北京 100029；2. 河南中医学院第一附属医院，河南郑州 450000

收稿日期:2011-09-05 修回日期:2011-12-22 出版日期:2012-09-05 发布日期:2012-09-05
通讯作者: 唐启盛,tangqisheng@263.net E-mail:tangqisheng@263.net；hyl_bj2008@sina.com
基金资助:
国家自然科学基金资助项目（30772804），北京中医药大学自主选题项目(2011JYBIIJS-028).

Effects of Yinao Jieyu Recipe in Intervening Cerebral Metabolism in Depressive-Like Rats Studied by In Vivo Magnetic Resonance Spectroscopy

HUANG Yu-Ling¹, TANG Qi-Sheng^1*, LI Ning², ZHU Chen-Jun¹

1. The Third Affiliate Hospital of Beijing University of Chinese Medicine, Beijing 100029, China; 
2. The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, China

Received:2011-09-05 Revised:2011-12-22 Online:2012-09-05 Published:2012-09-05
Contact: TANG Qi-Sheng,tangqisheng@263.net E-mail:tangqisheng@263.net；hyl_bj2008@sina.com
Supported by:
国家自然科学基金资助项目（30772804），北京中医药大学自主选题项目(2011JYBIIJS-028).

摘要/Abstract

摘要：

该文主要研究抑郁大鼠脑的¹H MRS变化及颐脑解郁方的干预作用. 将雄性Wistar大鼠随机分为正常组、模型组、西药组和中药组，每组5只. 正常组常规饲养，模型组、中药组和西药组给予21 d的慢性不可预知的温和应激. 应激结束中药组予颐脑解郁方、西药组予盐酸氟西汀进行干预. 干预结束后，通过检测左侧海马及前额叶皮质N-乙酰天门冬氨酸（NAA）、胆碱（Cho）、肌酸（Cr）等代谢物水平，分别计算NAA、Cho与Cr的比值，进而对脑组织代谢进行定性及定量分析. 得到：1. 海马区域：与正常组相比，模型组、西药组大鼠海马NAA/Cr降低（P<0.01），中药组大鼠NAA/Cr与模型组相比升高（P<0.05）；与正常组相比，模型组Cho/Cr升高（P<0.05），中药组Cho/Cr比模型组显著低（P<0.01）. 2. 前皮质区域：与正常组相比，模型组、西药组大鼠前皮质NAA/Cr降低（P<0.01），中药组大鼠NAA/Cr较模型组显著升高（P<0.01）；模型组、西药组Cho/Cr与正常组相比显著升高（P<0.01）；与模型组相比，中药组、西药组Cho/Cr降低（P<0.01，P<0.05）. 这些结果说明颐脑解郁方可改善大鼠海马和前皮质的物质代谢，推测其抗抑郁作用主要与调节脑组织异常代谢有关.

关键词: 磁共振波谱(MRS), 抑郁大鼠, 前皮质, 海马

Abstract:

This study investigated the metabolic change in brain of depression-like rats and the intervention effects of Yinao Jieyu recipe. Twenty rats were randomly allocated into normal group, depression group, fluoxetine group and Yinao Jieyu recipe group, with five rats in each group. The animals in the normal group were raised regularly. The animals in the other three groups were subjected to chronic unpredictable mild stress (CUMS) for 21 days. After the CUMS treatment, the animals in the depression group received saline treatment by gastrogavage for 6 weeks, and those in the fluoxetine group and Yinao Jieyu recipe group received fluoxetine and Jieyu recipe treatments, respectively, by gastrogavage for 6 weeks. In vivo ¹H MRS was performed to measure the changes of N-acetyl aspartate (NAA), choline (Cho) and creatine (Cr) levels in the left prefrontal cortex and hippocampus. Compared to the normal group, the depression group and the fluoxetine group showed significantly decreased NAA/Cr ratio in the hippocampus and frontal cortex. The Yinao Jieyu recipe group showed significantly higher NAA/Cr ratio in the hippocampus and prefrontal cortex relative to the depression group. The hippocampal Cho/Cr ratio in the depression group was found increased (P<0.05), while that in the Yinao Jieyu recipe group decreased (P<0.01), compared to the normal group. The prefrontal Cho/Cr ration in the depression group and fluoxetine group showed significant increases relative to the normal. The prefrontal Cho/Cr ratios in the Yinao Jieyu recipe group and fluoxetine group were significantly lower than that in the depression group. In conclusion, the Yinao Jieyu recipe appeared to have apparent anti-depression effects in rats subjected to CUMS.

Key words: magnetic resonance spectroscopy(MRS), depression , rat, prefrontal cortex, hippocampus

黄育玲, 唐启盛, 李宁, 朱晨军. 颐脑解郁方对抑郁大鼠脑¹H波谱的干预作用[J]. 波谱学杂志, 2012, 29(3): 379-387.

HUANG Yu-Ling, TANG Qi-Sheng, LI Ning, ZHU Chen-Jun. Effects of Yinao Jieyu Recipe in Intervening Cerebral Metabolism in Depressive-Like Rats Studied by In Vivo Magnetic Resonance Spectroscopy[J]. Chinese Journal of Magnetic Resonance, 2012, 29(3): 379-387.

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颐脑解郁方对抑郁大鼠脑¹H波谱的干预作用

Effects of Yinao Jieyu Recipe in Intervening Cerebral Metabolism in Depressive-Like Rats Studied by In Vivo Magnetic Resonance Spectroscopy

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