ESR自旋捕集技术的发展和在生物医学研究中的应用

摘要/Abstract

摘要：

ESR是研究自由基最直接和最有效的技术，但是这些自由基必须是相对稳定的，而且要达到一定浓度才能用ESR技术检测和研究. 而生物体系中产生的自由基大部分是不稳定的，这是常规ESR波谱仪无法检测的. 为了克服ESR技术的这一局限性，发展了自旋捕集技术，这是目前研究生物和医学体系中活泼自由基应用最多也是最成功的方法，每年都有新的自旋捕集剂合成和大量在生物医学应用的报道，为自由基生物医学的研究和发展做出了巨大贡献. 
作者建立和发展了捕捉超氧阴离子自由基、羟基自由基、脂质过氧化产生的脂类自由基、一氧化氮自由基和单线态氧及一氧化氮和氧自由基同时检测技术. 利用这些技术开展了细胞、组织产生自由基机理，天然抗氧化剂的筛选及一些重大疾病如炎症、心脏病、老年痴呆症、帕金森综合症，中风，辐射损伤，蛋白质氧化，植物光合系统中产生活性自由基和植物的发病机理研究中的应用，取得了一系列研究结果.

关键词: 电子自旋共振(ESR), 自旋捕集技术, 自由基, 生物学, 医学

Abstract:

ESR is the most direct and effective technique to detect free radicals, however because most free radicals in biological systems are not stable, it is difficult to detect the free radicals by normal ESR. In order to breakthrough this limit, the spin trapping technique has been developed and successfully applied to detect the free radicals in biological system. More new spin trapping agents and a lot of papers are published in the field every year, which promote the development of free radical biology and medicine. 
We have developed the technique of spin trapping technique to detect super oxide free radical, hydroxyl free radical, lipid free radical generated in lipid peroxidation, NO free radical and single oxygen in biological system. We have also developed a technique to simultaneously measure NO and oxygen free radicals generated from cell and tissues. Using this technique, we investigated the mechanism of free radical generation by cell and tissue, scavenging effects of natural antioxidants on free radicals, the free radical generation mechanisms in inflammation, heart disease, Alzheimer’s disease, Parkinson’s disease, stroke, radiation damage and plant disease. We have got many significant results and published many papers in international journals.

Key words: ESR, spin trapping, free radicals, biology, medicine

中图分类号:

O482.53

赵保路. ESR自旋捕集技术的发展和在生物医学研究中的应用[J]. 波谱学杂志, 2011, 28(1): 18-41.

ZHAO Bao-Lu. Developments and Applications of ESR Spin Trapping Technique in the Biological and Medical Studies[J]. Chinese Journal of Magnetic Resonance, 2011, 28(1): 18-41.

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