组蛋白、精蛋白及其与DNA相互作用的高分辨核磁共振研究

波谱学杂志 ›› 1985, Vol. 2 ›› Issue (4): 297-302.

• 研究论文 • 下一篇

组蛋白、精蛋白及其与DNA相互作用的高分辨核磁共振研究

袁传照¹, 吴军¹, 曾伟强¹, 戴培林², 夏熔², 张振芳²

1. 中国科学院遗传研究所;
2. 中国科学院武汉物理研究所

收稿日期:1984-09-18 修回日期:1985-07-12 出版日期:1985-12-05 发布日期:2018-01-23
基金资助:
中国科学院科学基金

THE HIGH RESOLUTION NMR STUDIES OF HISTONE,PRO-TAMINES AND THEIR INTERACTION WITH DNA

Yuan Chuanzhao¹, Wu Jun¹, Zeng Weiqiang¹, Dai Peilin², Xia Pong², Zhang Chenfang²

1. Institute of Genetics, Acaderaia Sinica;
2. Wuhan lastutite of Physics, Academia Sinica

Received:1984-09-18 Revised:1985-07-12 Online:1985-12-05 Published:2018-01-23

摘要/Abstract

摘要： 本文报道了采用超导核磁共振波谱仪研究组蛋白、精蛋白及其与DNA相互作用的新结果。
发现组蛋白与精蛋白的波谱有明显的差异;精蛋白的Arg-δCH₂质子峰高大而Zys-εCH₂质子峰低弱不见;精蛋白的VaL、ILe、Leu-CH₃质子峰强度也相对较为低弱。
当精蛋白与DNA相互作用,Arg-γCH₂质子峰变弱,但Arg-βCH₂和Arg-δCH₂质子峰则未见明显变化。

Abstract: In this paper we present some results of high resolution NMR studies of hi-stones, protamines and their interaction with DNA.
Differences were found in the spectral characteristics of histones and protamines.
The spectra of protamines showed that the Arg-δCH₂ peak was rather strong while the Lys-εCH₂ peak was rather weak. The intensity of proton resonances of Val, lie and Leu-CH₃ in protamines was relatively low and weak.
When protamines interacted with DNA. the intensity of proton peaks of Arg-δCH₂ became weak,but the Arg-δCH₂ and Arg-βCH₂ proton peaks of protamines did not show any obvious change.

袁传照, 吴军, 曾伟强, 戴培林, 夏熔, 张振芳. 组蛋白、精蛋白及其与DNA相互作用的高分辨核磁共振研究[J]. 波谱学杂志, 1985, 2(4): 297-302.

Yuan Chuanzhao, Wu Jun, Zeng Weiqiang, Dai Peilin, Xia Pong, Zhang Chenfang. THE HIGH RESOLUTION NMR STUDIES OF HISTONE,PRO-TAMINES AND THEIR INTERACTION WITH DNA[J]. Chinese Journal of Magnetic Resonance, 1985, 2(4): 297-302.

组蛋白、精蛋白及其与DNA相互作用的高分辨核磁共振研究

THE HIGH RESOLUTION NMR STUDIES OF HISTONE,PRO-TAMINES AND THEIR INTERACTION WITH DNA

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