顺磁金属离子与某些氨基酸分子络合的NMRT1法研究

波谱学杂志 ›› 1994, Vol. 11 ›› Issue (2): 173-180.

顺磁金属离子与某些氨基酸分子络合的NMRT₁法研究

胡皆汉¹, 屈铭¹, 韩秀文¹, 许永廷², 杨开海²

1. 中国科学院大连化学物理研究所, 大连 116023;
2. 辽宁师范大学分析测试中心

收稿日期:1993-02-01 修回日期:1993-05-12 出版日期:1994-06-05 发布日期:2018-01-20

THE STUDIES ON PARAMAGNETIC IONS COORDINATED TO SOME AMINO ACIDS BY NMR T₁ METHOD

Hu Jiehan¹, Qu Ming¹, Xiuwen¹, Xu Yongting², Yang Kaihai²

1. Dalian Institute or Chemical Physics, The Chinese Academy of Sciences, Dalian 116023;
2. Experimental Center, Liaoning Nonnal University, Dalian 116022

Received:1993-02-01 Revised:1993-05-12 Online:1994-06-05 Published:2018-01-20

摘要/Abstract

摘要： 本文研究了水溶液中微量顺磁离子Fe³⁺和Cu²⁺对蛋白质及多肽结构单元一氨基酸分子纵向弛豫时间T₁的影响,把文献已处理过的金属离子与分子只存在一种络合物种体系的公式,推广到了多个络合物种并存且存在交换的体系中,导出并验证了顺磁弛豫速率R_f。与金属离子浓度C_M成线性关系,较深入地讨论了斜率大小与络合能力、配体浓度的关系.

关键词: 顺磁离子, 顺磁弛豫, 络合能力

Abstract: The influence of paramagnetic ions, Fe³⁺ and Cu²⁺ on relaxation time T₁ of amino acids which are structural units of peptides has been investigated in this paper.The for-mula only for monospecies system of chemical molecule and metal ion has been ex-tended to polyspecies system and the system in which there are chemical exchanges. The linear relations of paramagnctic relaxation rate(R_1p) and concetration of metal ions (C_M) have been elucidated and demonstrated.The relations between the slope of curves and coordinating ability and concentration of ligands have been discussed.

Key words: Paramagnetic ions, Paramagnetic relaxation, Coordinating ability

胡皆汉, 屈铭, 韩秀文, 许永廷, 杨开海. 顺磁金属离子与某些氨基酸分子络合的NMRT₁法研究[J]. 波谱学杂志, 1994, 11(2): 173-180.

Hu Jiehan, Qu Ming, Xiuwen, Xu Yongting, Yang Kaihai. THE STUDIES ON PARAMAGNETIC IONS COORDINATED TO SOME AMINO ACIDS BY NMR T₁ METHOD[J]. Chinese Journal of Magnetic Resonance, 1994, 11(2): 173-180.

[1]	张乃霞, 吴娟, 张华群, 刘霞. NMR技术在蛋白质-蛋白质相互作用研究中的应用——泛素-蛋白水解酶体通路研究实例介绍[J]. 波谱学杂志, 2012, 29(2): 182-189.
[2]	刘主, 唐淳. 顺磁弛豫增强技术与蛋白质瞬态结构[J]. 波谱学杂志, 2011, 28(3): 301-316.

顺磁金属离子与某些氨基酸分子络合的NMRT₁法研究

THE STUDIES ON PARAMAGNETIC IONS COORDINATED TO SOME AMINO ACIDS BY NMR T₁ METHOD

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