四川九节龙中新五糖三萜皂苷结构的NMR研究

波谱学杂志 ›› 1999, Vol. 16 ›› Issue (5): 395-402.

四川九节龙中新五糖三萜皂苷结构的NMR研究

缪振春¹, 冯锐², 周永新¹, 李光玉², 张清华³

1. 军事医学科学院毒物药物研究所, 北京 100850;
2. 国家生物医学分析中心, 北京 100850;
3. 华西医科大学药学院, 成都 610064

收稿日期:1999-04-29 修回日期:1999-06-18 出版日期:1999-10-05 发布日期:2018-01-13
作者简介:缪振春,男,1940年出生,高工

NMR STUDY ON A NEW TRITERPENOID GLYCOSIDE OF ARDISIA PUSILLA

Miao Zhenchun¹, Feng Rui², Zhou Yongxin¹, Li Guangyi², Zhang Qinghua³

1. Institute of Pharmacology and Toxicology, Beijing 100850;
2. Academy of Military MedicalSciences, Beijing 100850;
3. Schoolof Pharmacy, West China University of Medical Sciences, Chengdu 610041

Received:1999-04-29 Revised:1999-06-18 Online:1999-10-05 Published:2018-01-13

摘要/Abstract

摘要： 从四川九节龙(Ardisia pusilla A.DC)的正丁醇提取物中分得一个三萜皂苷.经过测定,它为:3-O-[β-D-吡喃木糖(1→2)-β-D吡喃葡萄糖(1→4)] [β-D-吡喃葡萄糖(1→2)-β-D-吡喃葡萄糖(1→2)]-α-L-吡喃阿拉伯糖仙客来亭A,为一新五糖皂苷(1).糖体间和糖体与苷元间的连接顺序和连接位置采用一维SEMDY和旋转坐标系NOE差谱核磁共振新技术相结合的方法进行了研究.结果表明,这一方法用于测定寡糖链结构具有准确、简便、快速等特点,对化合物不需要进行化学降解或衍生化,糖基的重叠¹H NMR信号可以分辨和正确指定.

关键词: 九节龙, 五糖皂苷, 糖链结构测定, 旋转坐标系NOE差谱, 一维SEMDY谱

Abstract: A new triterpenoid saponin was isolated from Ardisia pusilla A. DC.which has improved immunological and antitumor and its structure established as 3-O-[β-D-xylopyranosyl (1→2) -β -D-glucopyranosyl(1→4)] [β -D-glucopyranosyl(1→2) -β-D-glucopyranosyl(1→2)]-α -L-arabinopyranosyl cyclamiretin A.The results showed that the chemical structure of oligosaccharide chains of the saponin could be elucidated by the combination of 1D-SEMDY and NOE difference spectroscopy in rotating frame techniques, without having recourse to chemical degration or modification. Complete assignments of severely overlapping proton resonance of the sugar residues were obtained.

Key words: Ardisia pusilla A.DC, Structural detemination of saponin, NOE difference spectroscopy in rotating frame, 1D-SEMDY

缪振春, 冯锐, 周永新, 李光玉, 张清华. 四川九节龙中新五糖三萜皂苷结构的NMR研究[J]. 波谱学杂志, 1999, 16(5): 395-402.

Miao Zhenchun, Feng Rui, Zhou Yongxin, Li Guangyi, Zhang Qinghua. NMR STUDY ON A NEW TRITERPENOID GLYCOSIDE OF ARDISIA PUSILLA[J]. Chinese Journal of Magnetic Resonance, 1999, 16(5): 395-402.

[1]	缪振春, 冯锐, 周永新, 魏锋. 日本续断中新五糖常春藤皂苷的结构和NMR研究[J]. 波谱学杂志, 1999, 16(4): 289-294.
[2]	缪振春, 冯锐, 周永新, 魏锋. 川续断中新七糖三萜皂苷的核磁共振研究[J]. 波谱学杂志, 1999, 16(1): 44-51.
[3]	缪振春, 冯锐, 李光玉, 杜泽涵. 旋转坐标系同核NOE差谱[J]. 波谱学杂志, 1997, 14(1): 83-88.