日本续断中新五糖常春藤皂苷的结构和NMR研究

波谱学杂志 ›› 1999, Vol. 16 ›› Issue (4): 289-294.

日本续断中新五糖常春藤皂苷的结构和NMR研究

缪振春¹, 冯锐², 周永新¹, 魏锋³

1. 军事医学科学院毒物药物研究所, 北京 100850;
2. 国家生物医学分析中心, 北京 100850;
3. 北京江中药物研究所, 北京 100050

收稿日期:1999-02-08 出版日期:1999-08-05 发布日期:2018-01-11
作者简介:缪振春,男,1940年出生,高级工程师

STRUCTURAL ANALYSIS OF OLIGOSACCHARIDE CHAIN OF A NEW GLYCOSIDE FROM DIPSOCUS JAPONICUS BY 1D-SEMDY AND ROE DIFFERENCE SPECTROSCOPY TECHNIQUES

Miao Zhenchun¹, Feng Rui², Zhou Yongxin¹, Wei Feng³

1. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850;
2. National Center of Biomedical Analysis, Academy of Military Medical Sciences, Beijing 100850;
3. Beijing Jiangzhong Pharmaceutical Institute, Beijing 100050

Received:1999-02-08 Online:1999-08-05 Published:2018-01-11

摘要/Abstract

摘要： 从日本续断根部的乙醇提取物中分得一个新的三萜皂苷.经过测定,它为:3-O-[β-D-吡喃葡萄糖(1→4)][α-L-吡喃鼠李糖(1→3)]-β-D-吡喃葡萄糖(1→3)-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖常春藤甙元.结果表明,采用一维SEMDY和旋转坐标NOE差谱核磁共振新技术相结合的方法测定这一类化合物的糖链结构不需要对化合物进行化学降解或衍生化,方法简便、快速,测定结果可靠,每个糖基的¹H NMR信号可以分辨和明确归属.

关键词: 日本续断, 五糖皂苷, 糖链结构测定, 旋转坐标NOE差谱, 一维SEMDY谱

Abstract: A new triterpenoid saponin was isolated from the ethanolic extract of the root of Dipsocus japonicus and its structure was established as 3-O-[β-D-glucopyrano-syl(1→4)] [α-L-rhamnopyranosyl(1→3)]-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranosyl-hederagenin. The results obtained showed that the chemical structure of oligosaccharide chain of the saponin could be elucidated by the combination of 1D-SEMDY and NOE difference spectroscopy in rotating frame techniques, without having recourse to chemical degration or modification. Complete assignments of severely overlapping proton resonance of the sugar residues were obtained.

Key words: Dipsocus japonicus, Structural determination of Oligosaccharide Chain, NOE difference spectroscopy in rotating frame, 1D SEMDY

缪振春, 冯锐, 周永新, 魏锋. 日本续断中新五糖常春藤皂苷的结构和NMR研究[J]. 波谱学杂志, 1999, 16(4): 289-294.

Miao Zhenchun, Feng Rui, Zhou Yongxin, Wei Feng. STRUCTURAL ANALYSIS OF OLIGOSACCHARIDE CHAIN OF A NEW GLYCOSIDE FROM DIPSOCUS JAPONICUS BY 1D-SEMDY AND ROE DIFFERENCE SPECTROSCOPY TECHNIQUES[J]. Chinese Journal of Magnetic Resonance, 1999, 16(4): 289-294.

[1]	缪振春, 冯锐, 周永新, 李光玉, 张清华. 四川九节龙中新五糖三萜皂苷结构的NMR研究[J]. 波谱学杂志, 1999, 16(5): 395-402.
[2]	缪振春, 冯锐, 周永新, 魏锋. 川续断中新七糖三萜皂苷的核磁共振研究[J]. 波谱学杂志, 1999, 16(1): 44-51.