牡丹不同根部位的代谢物分布

doi:10.11938/cjmr20150410

波谱学杂志 ›› 2015, Vol. 32 ›› Issue (4): 648-660.doi: 10.11938/cjmr20150410

牡丹不同根部位的代谢物分布

肖超妮，王培，马翠霞，荣泽铭，郑晓晖*

西北大学生命科学学院，陕西西安 710069

收稿日期:2015-10-16 修回日期:2015-11-09 出版日期:2015-12-05 发布日期:2015-12-05
作者简介:肖超妮(1975-)，女，陕西户县人，博士，主要从事中药学研究． *通讯联系人：郑晓晖，电话： 029-88302686， E-mail： zhengxh@nwu.edu.cn.
基金资助:
陕西省重点科技创新团队资助项目(2013KCT-24)

The Distribution of Metabolites in Different Root Parts from Tree Peony

XIAO Chao-ni， WANG Pei， MA Cui-xia， RONG Ze-ming， ZHENG Xiao-hui*

College of Life Sciences, Northwest University, Xi’an 710069, China

Received:2015-10-16 Revised:2015-11-09 Online:2015-12-05 Published:2015-12-05
About author:*Corresponding author： ZHENG Xiao-hui, Tel: +86-29-88302686, E-mail: zhengxh@nwu.edu.cn.
Supported by:
陕西省重点科技创新团队资助项目(2013KCT-24)

摘要/Abstract

摘要：

该文应用核磁共振（ NMR）和高效液相色谱-质谱（ HPLC-MS）技术系统分析了牡丹不同根部位的代谢物组成，共鉴定了包括蔗糖、苯乙酰类（ 4 种）、酚酸类（ 6 种）、单萜苷类（ 2 种）、黄酮类（ 1 种）和不饱和脂肪酸（ 2 种）共 16 种代谢物．在牡丹根中，丹皮酚以及丹皮酚苷等苯乙酰类成分和蔗糖的含量较高，而酚酸、黄酮和单萜苷类成分的含量较低．基于 NMR 的代谢组分析表明，牡丹不同根部位的代谢物组成具有明显差异：在二级侧根、一级侧根和主根中，丹皮酚及丹皮酚苷类的含量依次较高而蔗糖含量依次降低，这可能与不同根部位的植物生理有关；和侧根相比，主根中具有活性的代谢物含量较高，推测主根的品质优于侧根，这为市场上牡丹根皮的等级划分提供了重要的科学依据．

关键词: 核磁共振（ NMR）, 高效液相色谱-质谱（ HPLC-MS）, 代谢物, 根部位, 牡丹

Abstract:

The distribution of metabolites in the root bark of tree peony from different root parts was systematically analyzed using the combination of NMR and HPLC-MS for the first time. A total of 16 metabolites from their methanol extracts were simultaneously identified and quantified, including 1 primary metabolite (sucrose) and 15 secondary ones such as 4 acetophenones, 6 phenolics, 2 monoterpene glycosides, 1 flavonoid and 2 unsaturated fatty acids. The quantitative data indicated that there are the higher levels of acetophenones and sucrose, and the lower contents of phenolics, monoterpene glycosides, flavonoids and unsaturated fatty acids in the tree peony roots. NMR-based metabolomics revealed that the metabolite compositions were found to be different among the three root parts. The increasing levels of acetophenones and decreasing level of sucrose were observed in second lateral root, the first lateral and axial root in order. The axial roots have higher quality than the lateral roots in tree peony due to the accumulation of bioactive secondary metabolites associated with plant physiology. These findings provided important scientific evidence for grading the root bark of tree peony on the general market.

Key words: NMR, HPLC-MS, metabolite, root part, tree peony

中图分类号:

O482.53

肖超妮，王培，马翠霞，荣泽铭，郑晓晖*. 牡丹不同根部位的代谢物分布[J]. 波谱学杂志, 2015, 32(4): 648-660.

XIAO Chao-ni， WANG Pei， MA Cui-xia， RONG Ze-ming， ZHENG Xiao-hui*. The Distribution of Metabolites in Different Root Parts from Tree Peony
[J]. Chinese Journal of Magnetic Resonance, 2015, 32(4): 648-660.

参考文献

[1] Pharmacopoeia Commission of People’s Republic of China(国家药典委员会). Pharmacopoeia of People’s Republic of China (中华人民共和国药典)[M]. Beijing(北京): Chemical Industry Press(化学工业出版社), 2010.

[2] Hu S L, Shen G, Zhao W G, et al. Paeonol, the main active principles of Paeonia moutan, ameliorates alcoholic steatohepatitis in mice[J]. J Ethnopharmacol, 2010, 128(1): 100－106.

[3] Fu P K, Yang C Y, Tsai T H, et al. Moutan cortex radicis improves lipopolysaccharide-induced acute lung injury in rats through anti-inflammation[J]. Phytomedicine, 2012, 19(13): 1 206－1 215.

[4] Yun C S, Choi Y G, Jeong M Y, et al. Moutan cortex radicis inhibits inflammatory changes of gene expression in lipopolysaccharide-stimulated gingival fibroblasts[J]. J Nat Med, 2013, 67(3): 576－589.

[5] Park J, Kim H Y, Lee S M. Protective effects of Moutan cortex radicis against acute hepatotoxicity[J]. Afr J Tradit Complem Alterna Med, 2011, 8(5): 220－225.

[6] Kim H G, Park G, Piao Y, et al. Effects of the root bark of Paeonia suffruticosa on mitochondria-mediated neuroprotection in an MPTP-induced model of Parkinson's disease[J]. Food Chem Toxicol, 2014, 65: 293－300.

[7] He C N, Peng Y, Zhang Y C, et al. Phytochemical and biological studies of Paeoniaceae[J]. Chem Biodivers, 2010, 7: 805－838.

[8] Zha L P, Cheng M E, Peng H S. Identification of ages and determination of paeoniflorin in roots of Paeonia lactiflora Pall. From four producing areas based on growth rings[J]. Microsc Res Techniq, 2012, 75(9): 1 191－1 196.

[9] Ding Y, Wu E Q, Chen J B, et al. Quality evaluation of Moutan cortex radicis using multiple component analysis by high performance liquid chromatography[J]. B Kor Chem Soc, 2009, 30(10): 2 240－2 244.

[10] Long Quan-jiang(龙全江), Wang Xiao-ge(王晓阁), Zhou Zhou(周宙), et al. Study on the influence of the contents of paeonol in the cutting process of fresh Cortex moutan(趁鲜切制法对牡丹皮饮片中丹皮酚含量的影响研究)[J]. Journal of Chinese Medicinal Materials(中药材), 2012, 35(6): 883－886.

[11] Sun Qing-lei(孙庆雷), Zhao Hong-xia(赵红霞), Lin Yun-liang(林云良), et al. ¹H NMR fringerprints of Scutellaria Baicalensis(黄芩的¹H NMR指纹图谱研究)[J]. Chinese J Magn Reson(波谱学杂志), 2007, 24(2): 163－168.

[12] Chen Bo(陈波), Kang Hai-ning(康海宁), Han Chao(韩超), et al. Applications of NMR spectroscopy and pattern recognition in food analysis (NMR指纹图谱与模式识别方法在食物分析中的应用)[J]. Chinese J Magn Reson(波谱学杂志), 2006, 23(3): 397－407.

[13] Dai H, Xiao C N, Liu H B, et al. Combined NMR and LC-MS analysis reveals the metabonomic changes in Salvia miltiorrhiza Bunge induced by water depletion[J]. J Proteome Res, 2010, 9: 1 460－1 475.

[14] Herve Du P C, Imberty A, Roques N, et al. Conformational behavior of sucrose and its deoxy analogue in water as determined by NMR and molecular modeling[J]. J Am Chem Soc, 1991, 113(10): 3 720－3 727.

[15] Kwon D Y, Song H N, Yoon S H. Synthesis of medium-chain glycerides by lipase in organic solvent[J]. J Am Oil Chem Soc, 1996, 73(11): 1 521－1 525.

[16] Kuwajima H, Shibano N, Baba T, et al. An acetophenone glycoside from Exacum affine[J]. Phytochemistry, 1996, 41(1): 289－292.

[17] Yu J, Lang H Y, Xiao P G. A new compound, apiopaeonoside, isolated from the root of Paeonia suffruticosa[J]. Acta Pharm Sin, 1986, 21(3): 191－197.

[18] Ha D T, Trung T N, HienT T, et al.Selected compounds derived from Moutan Cortex stimulated glucose uptake and glycogen synthesis via AMPK activation in human HepG2 cells[J]. J Ethnopharmacol, 2010, 131(2): 417－424.

[19] Peungvicha P, Temsiririrkkul R, Prasain J K, et al. 4-Hydroxybenzoic acid: a hypoglycemic constituent of aqueous extract of Pandanus odorus root[J]. J Ethnopharmacol, 1998, 62(1): 79－84.

[20] Lee S C, Kwon Y S, Son K H, et al. Antioxidative constituents from Paeonia lactiflora[J]. Arch Pharm Res, 2005, 28(7): 775－783.

[21] Puppala M, Ponder J, Suryanarayana P, et al. The isolation and characterization of beta-glucogallin as a novel aldose reductase inhibitor from Emblica officinalis[J]. Plos One, 2012, 7(4): e31399.

[22] Beretta G, Artali R, Caneva E, et al. Conformation of the tridimensional structure of 1, 2, 3, 4, 6-pentagalloyl-beta-D- glucopyranose (PGG) by H-1 NMR, NOESY and theoretical study and membrane interaction in a simulated phospholipid bilayer: a first insight[J]. Magn Reson Chem, 2010, 49(3): 132－136.

[23] Piao X S, Piao X L, Kim H Y, et al. Antioxidative activity of geranium (Pelargonium inquinans Ait) and its active component, 1, 2, 3, 4, 6-penta-O-galloyl-beta-D-glucose[J]. Phytother Res, 2008, 22(4): 534－538.

[24] Qi S H,Wu D G, Ma Y B, et al. A novel flavane carapa guianensis[J]. Acta Bot Sin, 2013, 45(9): 1 129－1 133.

[25] Drew S W, Demain A L. Effect of primary metabolites on secondary metabolism[J]. Annu Rev Microbiol, 1977, 31: 343－356.

[26] Wang X, Cheng C, Sun Q L, et al. Isolation and purification of four flavonoid constituents from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography[J]. J Chromatogra A, 2005, 1 075(1-2): 127－131.

[27] Li C, Du H, Wang L, et al. Flavonoid composition and antioxidant activity of tree peony (Paeonia Section Moutan) yellow flowers[J]. J Agric Food Chem, 2009, 57(18): 8 496－8 503.

[28] Baetz U, Martinoia E. Root exudates: the hidden part of plant defense[J]. Trends Plant Sci, 2013, 19(2): 90－98.

牡丹不同根部位的代谢物分布

The Distribution of Metabolites in Different Root Parts from Tree Peony

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价

[1]	刘富发, 杨晓艳, 豪富华, 王玉兰, 唐惠儒. 莽草酸途径中26种代谢物的NMR谱归属[J]. 波谱学杂志, 2017, 34(3): 311-322.
[2]	周宁, 杜祥博, 杨莉, 许怡, 孙博, 张琪, 戴二黑, 颜贤忠, 董方霆. 不同病程的结核病患者的血浆代谢组学研究[J]. 波谱学杂志, 2016, 33(2): 224-235.
[3]	邵巍1，顾金苹1，黄彩华2，黄子成1，季天海1，林东海1. NMR 分析胶质瘤细胞系CHG5 和U87 的代谢轮廓[J]. 波谱学杂志, 2014, 31(1): 40-48.
[4]	冯越1,2 ; 张许1 ; 刘买利1 . 代谢组学方法分析鸡胚胎发育过程中脑代谢物 [J]. 波谱学杂志, 2009, 26(1): 27-36.
[5]	李雪1，2 ；蓝文贤1，2 ；朱航1，2 ；刘买利1 ；张许1 . pH对血清影响的¹H NMR研究[J]. 波谱学杂志, 2008, 25(4): 494-503.
[6]	Lindon J C, Holmes E, Nicholson J K. 基于NMR的代谢组学方法最新进展及应用[J]. 波谱学杂志, 2006, 23(1): 101-127.
[7]	冯越1,2，常建华1，张许2，刘买利2*. 鸡胚羊水的核磁共振波谱分析[J]. 波谱学杂志, 2002, 19(2): 137-142.
[8]	翟纯. LC-NMR联用的现状与展望[J]. 波谱学杂志, 1996, 13(4): 403-410.