波谱学杂志

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内源性蛋白质分子成像的研究进展

周进元1,2*, 洪晓华1,3   

  1. 1. 约翰霍普金斯大学 放射系 磁共振研究部, 美国  马里兰州  巴尔的摩市  21287;
    2. F.M. Kirby脑功能成像研究中心, 美国  马里兰州  巴尔的摩市  21205;
    3. 华中科技大学 同济医学院 附属协和医院肿瘤科, 湖北 武汉 430020
  • 收稿日期:2012-06-04 修回日期:2012-07-09 出版日期:2013-09-05 发布日期:2013-09-05
  • 作者简介:周进元,1962年8月出生.1983年毕业于福建师范大学物理系,获理学学士学位.1996年毕业于中国科学院武汉物理研究所(现中国科学院武汉物理与数学研究所),获理学博士学位, 同年获中国科学院院长奖学金特别奖. 2012年获Academy of Radiology Research知名研究者奖. 现任美国约翰霍普金斯大学医学院放射系副教授,主要研究方向为活体磁共振成像方法及其在临床中的应用. 电话:+01-410-9557491, E-mail: jzhou@mri.jhu.edu.
  • 基金资助:

    National Natural Science Foundation of China (81128006) and US National Institutes of Health (R01EB009731, R21EB015555).

Molecular Imaging Using Endogenous Cellular Proteins

ZHOU Jin-yuan1,2*, HONG Xiao-hua1,3   

  1. 1. Division of MR Research, Department of Radiology, Johns Hopkins University, Baltimore, MD 21287, USA;
    2. F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD 21205, USA;
    3. Department of Oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430020, China
  • Received:2012-06-04 Revised:2012-07-09 Online:2013-09-05 Published:2013-09-05
  • About author:Zhou Jin-yuan(1962-), born in Nan'an, Fujian, Ph.D., Associate Professor of Radiology, Research interest: In vivo MRI. Tel: +01-410-9557491, E-mail: jzhou@mri.jhu.edu.
  • Supported by:

    National Natural Science Foundation of China (81128006) and US National Institutes of Health (R01EB009731, R21EB015555).

摘要:

酰氨质子转移(amide proton transfer, APT)成像是一种新的分子MRI技术,它可用来测量组织中内源性蛋白质. 理论上,APT-MRI信号强度主要取决于游离蛋白质的酰氨质子浓度以及交换速度,而酰氨质子交换速度与组织pH有关. 因此,APT-MRI技术已经被用于无创性中风pH成像(通常pH降低)和肿瘤蛋白质含量成像(通常蛋白质量提高). 近期对大鼠的实验表明,APT-MRI技术可用来区分放射性坏死和胶质瘤. 该综述文章简要地介绍了APT成像的基本原理以及它在动物模型与临床中风和肿瘤成像中的应用.

关键词: 酰氨质子转移, pH成像, 分子成像, 中风, 脑瘤

Abstract:

Amide proton transfer (APT) imaging is a novel molecular MRI technique that generates image contrast based on endogenous cellular proteins in tissue. Theoretically, the APT-MRI signal depends primarily on the mobile amide proton concentration and amide proton exchange rates (which are related to tissue pH). The APT technique has been used for non-invasive pH imaging in stroke (where pH drops) and protein content imaging in tumor (where many proteins are overexpressed). It has been demonstrated recently in animal models that the APT-MRI signal is also a unique imaging biomarker to distinguish between radiation necrosis and active tumor. In this paper, we will briefly introduce the basic principles of APT imaging and review its applications in stroke and brain tumor studies in animal models and patients.

Key words: amide proton transfer (APT), pH imging, molecular imaging, stroke, brain tumor

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