大鼠脑缺血边缘区代谢物1H-MRS的研究

波谱学杂志

• 研究论文 • 下一篇

大鼠脑缺血边缘区代谢物¹H-MRS的研究

易黎1,2，卢广1，刘买利1，张苏明2

1.中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室，武汉 43007 1；
2.华中科技大学同济医学院附属同济医院，武汉 430030

收稿日期:2001-07-11 修回日期:2001-11-15 出版日期:2002-03-05 发布日期:2002-03-05
作者简介:易黎（1970-），男，博士研究生.
基金资助:
国家自然科学基金重点资助项目（39730170）；国家自然科学基金资助项目（39770810）；国家自然科学基金资助项目（30070825）.

¹H-MRS STUDY OF THE METABOLITE ON THE BORDER ZONE OF ACUTE CEREBRAL ISCHEMIA

YI Li1,2, LU Guang1, LIU Mai-li1, ZHANG Su-ming2

1.State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan 430071, China;
2.Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Received:2001-07-11 Revised:2001-11-15 Online:2002-03-05 Published:2002-03-05
Supported by:
国家自然科学基金重点资助项目（39730170）；国家自然科学基金资助项目（39770810）；国家自然科学基金资助项目（30070825）.

摘要/Abstract

摘要：

目的利用不同的局灶性脑缺血模型，评价急性缺血后磁共振质子波谱（¹H-MRS）测定缺血后边缘区脑组织代谢和生物能量变化的时空规律. 为判定急性缺血预后，进行有效的溶栓治疗提供有价值的生物化学信息. 方法健康Sprague-Dawly大鼠9只，雌雄不拘，随机分为两组. A组（4只），自体血栓栓塞1 h；B组（5只），线栓法栓塞1 h. 分别于栓塞后30、40、50、60 min进行¹HMRS检查，相对含量分析兴趣区（regio ns of interest, ROIs）氮-乙酰天门冬氨酸（NAA）、胆碱（Cho）和乳酸（Lac）等代谢产物的变化. 结果以NAA、Cho、Lac与磷酸肌酸和肌酸（PCr+Cr）的谱峰积分面积比值为判断标准，上述各代谢产物在兴趣区内于缺血后1 h内逐渐下降. 其中缺血后60 min，Cho/ （P Cr+Cr）、NAA/（PCr+Cr）及Lac/（PCr+Cr）的比值与缺血后50 min的比值统计学有显著性差异（P<0.05）. 结论 ¹H-MRS技术为研究急性缺血性卒中后脑细胞代谢、生化能量状态提供了一个无创性、直接性、综合性的研究工具.

关键词: 磁共振质子波谱, 脑缺血

Abstract:

Objective The use of different models of focal cerebral ischemia to appraise the time-space rules of ¹H-MRS on measuring metabolism and energy changes in the post-ischemia brain tissue provides the clinical doctors with valuable informati on to judge the prognosis and carry out more effective therapy from the biochemi cal point of view. Methods 9 healthy Sprague-Dawly rats(indiscrimi nate the sex) was divided into two groups randomly. Group A (4 rats), occluded with self-thrombus for 1 h; Group B (5 rats), occluded with thread-emboli fo r 1 h. The ¹H-MRS was examined in 30, 40, 50, 60 min after occlusion respectively, and the metabolic changes of NAA, C ho and Lac in the reg ions of interest in partly-fixed quantity were analyzed. Results Set the resonance intregrated area ratio of NAA, Cho, Lac to Pcr+Cr as the criterion, the values of a ll the metabolites declined gradually within 1 h after ischemia. Especially, the ratio of Cho/（Pcr+Cr）、NAA/（Pcr+Cr）and Lac/（Pcr+Cr）in 60 min has significant difference from that in 50 min (P<0.05). Conclusions Magnetic Reson ance Proton Spectroscopy is a good research tool, which is straightforward, comp rehe nsive and no damage, for the study of cellular metabolism and the status of the biochemical energy in acute ischemia stroke.

Key words: magnetic resonance proton spectroscopy, ischemia

中图分类号:

R816.1

易黎1,2，卢广1，刘买利1，张苏明2. 大鼠脑缺血边缘区代谢物¹H-MRS的研究[J]. 波谱学杂志.

YI Li1,2, LU Guang1, LIU Mai-li1, ZHANG Su-ming2. ¹H-MRS STUDY OF THE METABOLITE ON THE BORDER ZONE OF ACUTE CEREBRAL ISCHEMIA[J]. Chinese Journal of Magnetic Resonance.

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大鼠脑缺血边缘区代谢物¹H-MRS的研究

¹H-MRS STUDY OF THE METABOLITE ON THE BORDER ZONE OF ACUTE CEREBRAL ISCHEMIA

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