人脑GABA磁共振波谱测量中大分子影响的研究

doi:10.11938/cjmr20160411

波谱学杂志 ›› 2016, Vol. 33 ›› Issue (4): 618-626.doi: 10.11938/cjmr20160411

人脑GABA磁共振波谱测量中大分子影响的研究

陈梅泞, 王前锋, 李改英, 张竹伟, 陈录广, 李建奇

华东师范大学物理系, 上海市磁共振重点实验室, 上海 200062

收稿日期:2016-04-11 修回日期:2016-10-24 出版日期:2016-12-05 发布日期:2016-12-05
通讯作者: 李建奇,电话:021-62223871,E-mail:jqli@phy.ecnu.edu.cn E-mail:jqli@phy.ecnu.edu.cn
作者简介:陈梅泞(1990-),女,四川自贡人,硕士研究生,无线电物理专业
基金资助:
国家自然科学基金资助项目（81271533），国家社科基金重大课题（15ZDB016）.

Effects of Macromolecules on Cerebral GABA Measurements Using Magnetic Resonance Spectroscopy

CHEN Mei-ning, WANG Qian-feng, LI Gai-ying, ZHANG Zhu-wei, CHEN Lu-guang, LI Jian-qi

Shanghai Key Laboratory of Magnetic Resonance, Department of Physics, East China Normal University, Shanghai 200062, China

Received:2016-04-11 Revised:2016-10-24 Online:2016-12-05 Published:2016-12-05

摘要/Abstract

摘要： J差分谱编辑技术已广泛用于人脑γ-氨基丁酸（γ-amino butyric acid，GABA）的检测，利用MEGA-PRESS序列可以有效编辑GABA在δ_H 3.02处的信号.由于相近的化学位移和J耦合作用，大分子（macromolecule，MM）在δ_H 3.00的信号也同时被编辑，因此测量得到的GABA信号中包含一部分MM信号（GABA+MM，简称GABA+）.对称谱编辑技术可以有效抑制大分子，但该方法对编辑脉冲的频率选择性要求较高，因此对称谱编辑技术中编辑脉冲持续时间一般较长.该研究将MEGA-PRESS序列中编辑脉冲持续时间由14 ms增加到20 ms，回波时间（echo time，TE）由68 ms增加到80 ms，分别测量GABA+与对称谱编辑技术抑制大分子后的GABA（简称GABA）.结果发现，在较长编辑脉冲作用时间和较长TE条件下，GABA比GABA+含量低27%；长编辑脉冲持续时间可以有效提高编辑脉冲的频率选择性，更好地实现对称谱编辑技术抑制大分子，有助于分析人脑GABA含量测定中大分子信号干扰的影响.纯GABA含量测量，有助于更准确地分析GABA在人脑中的代谢过程，以及GABA含量与各种疾病和功能认知反应之间的相关性.

关键词: 大分子, 对称谱编辑技术, J差分谱编辑技术, γ-氨基丁酸(GABA)

Abstract: The MEGA-PRESS sequence has been widely used to measure the δ_H 3.02 γ-amino butyric acid (GABA) signal by J-difference editing. However, the sequence cannot eliminate the macromolecule signals at the same chemical shift completely. Symmetrical editing can be applied to suppress the macromolecule signals. However, the approach is rarely applied at field strength of 3 T due to insufficient frequency selectivity of the editing pulse. In this study, GABA+(i.e., GABA+macromolecules) and macromolecule-suppressed GABA signals in the occipital lobe of human subjects were measured with symmetrical editing at 3 T. The duration of the editing pulse was increased from 14 ms to 20 ms to improve frequency selectivity, and echo time (TE) from 68 ms to 80 ms. It was found that the fraction of the total signal retained following macromolecule suppression ([GABA]/[GABA+]) was 0.73. It is concluded that symmetric macromoleculesuppressed editing can be used to acquire macromolecule-suppressed GABA signals, and may serve as a better tool to assess inter-individual differences in cerebral GABA level.

Key words: GABA, J-difference editing, symmetric macromoleculesuppressed editing, macromolecules

中图分类号:

O482.53

陈梅泞, 王前锋, 李改英, 张竹伟, 陈录广, 李建奇. 人脑GABA磁共振波谱测量中大分子影响的研究[J]. 波谱学杂志, 2016, 33(4): 618-626.

CHEN Mei-ning, WANG Qian-feng, LI Gai-ying, ZHANG Zhu-wei, CHEN Lu-guang, LI Jian-qi. Effects of Macromolecules on Cerebral GABA Measurements Using Magnetic Resonance Spectroscopy[J]. Chinese Journal of Magnetic Resonance, 2016, 33(4): 618-626.

参考文献

[1] Chen Yao-wen(陈耀文), Shen Zhi-wei(沈智威), Su Ji-wei(宿吉伟), et al. Quantitative measurement of γ-aminobutyric acid concentration in vivo by magnetic resonance spectroscopy:A review(MRS定量检测活体γ-氨基丁酸浓度的研究现状)[J]. Chinese J Magn Reson(波谱学杂志), 2006, 23(3):409-417.
[2] Whittington M A, Traub R D, Jefferys J G. Synchronized oscillations in interneuron networks driven by metabotropic glutamate receptor activation[J]. Nature, 1995, 373(6515):612-615.
[3] Simister R J, Mclean M A, Barker G J, et al. Proton MR spectroscopy of metabolite concentrations in temporal lobe epilepsy and effect of temporal lobe resection[J]. Epilepsy Res, 2009, 83(2):168-176.
[4] Bielau H, Steiner J, Mawrin C, et al. Dysregulation of GABAergic neurotransmission in mood disorders:a postmortem study[J]. Ann N Y Acad Sci, 2007, 1096:157-169.
[5] Farzan F, Barr M S, Levinson A J, et al. Evidence for gamma inhibition deficits in the dorsolateral prefrontal cortex of patients with schizophrenia[J]. Brain, 2010, 133(5):1505-1514.
[6] Sumner P, Edden R A, Bompas A, et al. More GABA, less distraction:a neurochemical predictor of motor decision speed[J]. Nat Neurosci, 2010, 13(7):825-827.
[7] Puts N A, Edden R A, Evans C J, et al. Regionally specific human GABA concentration correlates with tactile discrimination thresholds[J]. J Neurosci, 2011, 31(46):16556-16560.
[8] Edden R A, Muthukumaraswamy S D, Freeman T C, et al. Orientation discrimination performance is predicted by GABA concentration and gamma oscillation frequency in human primary visual cortex[J]. J Neurosci, 2009, 29(50):15721-15726.
[9] Boy F, Evans C J, Edden R A, et al. Dorsolateral prefrontal gamma-aminobutyric acid in men predicts individual differences in rash impulsivity[J]. Biol Psychiatry, 2011, 70(9):866-872.
[10] Donahue M J, Near J, Blicher J U, et al. Baseline GABA concentration and fMRI response[J]. Neuroimage, 2010, 53(2):392-398.
[11] Northoff G, Walter M, Schulte R F, et al. GABA concentrations in the human anterior cingulate cortex predict negative BOLD responses in fMRI[J]. Nat Neurosci, 2007, 10(12):1515-1517.
[12] Behar K L, Ogino T. Characterization of macromolecule resonances in the ¹H NMR spectrum of rat brain[J]. Magn Reson Med, 1993, 30(1):38-44.
[13] Henry P G, Dautry C, Hantraye P, et al. Brain GABA editing without macromolecule contamination[J]. Magn Reson Med, 2001, 45(3):517-520.
[14] Aufhaus E, Weber-fahr W, Sack M, et al. Absence of changes in GABA concentrations with age and gender in the human anterior cingulate cortex:a MEGA-PRESS study with symmetric editing pulse frequencies for macromolecule suppression[J]. Magn Reson Med, 2013, 69(2):317-320.
[15] Edden R A, Puts N A, Barker P B. Macromolecule-suppressed GABA-edited magnetic resonance spectroscopy at 3 T[J]. Magn Reson Med, 2012, 68(3):657-661.
[16] Mikkelsen M, Singh K D, Sumner P, et al. Comparison of the repeatability of GABA-edited magnetic resonance spectroscopy with and without macromolecule suppression[J]. Magn Reson Med, 2016, 75(3):946-953.
[17] Chen Lu-guang(陈录广), Li Jian-qi(李建奇), Wang Qian-feng(王前锋). Detection of GABA in human brain on clinical 3 T MRI scanner(人脑GABA测量在临床3 T磁共振成像系统上的实现)[J]. Chinese J Magn Reson(波谱学杂志), 2013, 30(3):345-353.
[18] Mescher M, Merkle H, Kirsch J, et al. Simultaneous in vivo spectral editing and water suppression[J]. NMR Biomed, 1998, 11(6):266-272.
[19] Harris A D, Puts N A, Edden R A. Tissue correction for GABA-edited MRS:Considerations of voxel composition, tissue segmentation, and tissue relaxations[J]. J Magn Reson Imaging, 2015, 42(5):1431-1440.
[20] Mandal P K. In vivo proton magnetic resonance spectroscopic signal processing for the absolute quantitation of brain metabolites[J]. Eur J Radiol, 2012, 81(4):653-664.
[21] Cabanes E, Confort-Gouny S, Le F Y, et al. Optimization of residual water signal removal by HLSVD on simulated short echo time proton MR spectra of the human brain[J]. J Magn Reson, 2001, 150(2):116-125.
[22] Vanhamme L, van Den Boogaart A, van Huffel S. Improved method for accurate and efficient quantification of MRS data with use of prior knowledge[J]. J Magn Reson, 1997, 129(1):35-43.
[23] Ashburner J, Friston K J. Unified segmentation[J]. Neuroimage, 2005, 26(3):839-851.
[24] Rothman D L, Petroff O A, Behar K L, et al. Localized ¹H NMR measurements of gamma-aminobutyric acid in human brain in vivo[J]. Proc Natl Acad Sci U S A, 1993, 90(12):5662-5666.
[25] Edden R A, Intrapiromkul J, Zhu H, et al. Measuring T₂ in vivo with J-difference editing:application to GABA at 3 tesla[J]. J Magn Reson Imaging, 2012, 35(1):229-234.
[26] Near J, Simpson R, Cowen P, et al. Efficient gamma-aminobutyric acid editing at 3 T without macromolecule contamination:MEGA-SPECIAL[J]. NMR Biomed, 2011, 24(10):1277-1285.

人脑GABA磁共振波谱测量中大分子影响的研究

Effects of Macromolecules on Cerebral GABA Measurements Using Magnetic Resonance Spectroscopy

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