HdeA在细菌外膜囊泡环境下的原位NMR研究

王欢,陶志清,姜国胜,张许,王冠,禾立春,刘买利

In situ Investigation of HdeA in Bacterial Outer Membrane Vesicles Using NMR Spectroscopy

WANG Huan,TAO Zhiqing,JIANG Guosheng,ZHANG Xu,WANG Guan,HE Lichun,LIU Maili

图5 HdeA在OMVs中残基的化学位移扰动.(a) pH 2.5和pH 6.5条件下，HdeA在OMVs环境中与在稀溶液中各残基的CSP，其中*号表示该残基由于峰重叠导致未能得到可信的CSP数据；(b) HdeA的单体结构，红色标注部分为CSP值变化较大的残基

Fig. 5 The CSP of residues of HdeA in OMVs. (a) Chemical shift perturbation difference of each residue of HdeA in the OMVs environment and dilute solution at pH 2.5 and pH 6.5, where * indicates that no credible CSP data could be obtained for this residue due to peak overlap; (b) Monomer structure of HdeA, in which residues with large variation in CSP values are marked in red