HdeA在细菌外膜囊泡环境下的原位NMR研究

王欢,陶志清,姜国胜,张许,王冠,禾立春,刘买利

In situ Investigation of HdeA in Bacterial Outer Membrane Vesicles Using NMR Spectroscopy

WANG Huan,TAO Zhiqing,JIANG Guosheng,ZHANG Xu,WANG Guan,HE Lichun,LIU Maili

图2 (a) HdeA-OMVs样品在SDS-PAGE电泳胶图，箭头标注条带（~10 kDa）为HdeA，其余条带为OMVs中的周质蛋白或外膜蛋白；(b)培养基上清中的HdeA蛋白经过SEC分离后的液相色谱图，在图中约68 mL的位置为HdeA的二体峰

Fig. 2 (a) SDS-PAGE gel plot of HdeA-OMVs samples, the arrow marked band (~10 kDa) is HdeA, and the remaining bands are pericytoplasmic proteins or outer membrane proteins in OMVs; (b) Liquid chromatogram of the HdeA from medium supernatant separated after size exclusion chromatography, in which the peak of the dimer HdeA is located at about 68 mL