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细胞色素c甲硫氨酸氧化机制的NMR研究
赵蓓蓓1,2,占建华1,2,胡琴1,2,朱勤俊1,刘买利1,2,3,4,张许1,2,3,4,*()
NMR Study on the Mechanism of Cytochrome c Methionine Oxidation
ZHAO Beibei1,2,ZHAN Jianhua1,2,HU Qin1,2,ZHU Qinjun1,LIU Maili1,2,3,4,ZHANG Xu1,2,3,4,*()

图5. 不同氧化程度的Met甲基选择性13C标记细胞色素c的1H-13C HSQC和圆二色谱. (a) 25 μmol/L Met甲基选择性13C标记细胞色素的1H-13C HSQC谱；(b)向图(a)样品中加入200 μmol/L H2O2后的1H-13C HSQC谱；(c)不同浓度H2O2存在时，Met甲基选择性13C标记细胞色素c的1H-13C HSQC谱中不同Met甲基信号的变化；(d)不同浓度H2O2存在时，Met甲基选择性13C标记细胞色素c的不同Met甲基信号积分变化；(e)不同浓度H2O2存在时，Met甲基选择性13C标记细胞色素c的圆二色谱

Fig. 5. 1H-13C HSQC spectra and CD spectra of methyl-13C methionine labeled cytochrome c in addition with hydrogen peroxide with different concentrations. (a) 1H-13C HSQC spectrum of 25 μmol/L methyl- 13C methionine labeled cytochrome c; (b) 1H-13C HSQC spectrum of sample in Fig. (a) in addition with 200 μmol/L H2O2; (c) Methyl signals in the 1H-13C HSQC spectra of methyl-13C methionine labeled cytochrome c in addition with different concentrations of hydrogen peroxide; (d) The methionine integrals of methyl-13C methionine labeled cytochrome c change with the concentrations of added hydrogen peroxide; (e) CD spectra of methyl-13C methionine labeled cytochrome c in addition with different concentrations of hydrogen peroxide