细胞色素c甲硫氨酸氧化机制的NMR研究
赵蓓蓓1,2,占建华1,2,胡琴1,2,朱勤俊1,刘买利1,2,3,4,张许1,2,3,4,*(
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NMR Study on the Mechanism of Cytochrome c Methionine Oxidation
ZHAO Beibei1,2,ZHAN Jianhua1,2,HU Qin1,2,ZHU Qinjun1,LIU Maili1,2,3,4,ZHANG Xu1,2,3,4,*()
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图3. 不同状态的Met甲基13C标记的细胞色素c的1H-13C HSQC谱图. (a) 0.1 mmol/L Met甲基选择性13C标记的细胞色素c；(b)向(a)图样品中加入1 mmol/L（十倍浓度）铁氰化钾；(c)向(a)图样品中加入1 mmol/L（十倍浓度）抗坏血酸钠
Fig. 3. 1H-13C HSQC spectra of methyl-13C methionine labeled cytochrome c under different states. (a) 0.1 mmol/L methyl- 13C methionine labeled cytochrome c; (b) Sample in Fig. (a) with addition of 1 mmol/L potassium ferricyanide; (c) Sample in Fig. (a) with addition of 1 mmol/L sodium ascorbate
