溶液中ATAD2溴结构域聚集行为的研究
The Aggregation of ATAD2 Bromodomain in Solution
溶液中ATAD2溴结构域聚集行为的研究 |
| 王远方,王小花,舒畅,张许,刘买利,曾丹云 |
|
The Aggregation of ATAD2 Bromodomain in Solution |
| WANG Yuanfang,WANG Xiaohua,SHU Chang,ZHANG Xu,LIU Maili,ZENG Danyun |
| 图6 (a) C1101A突变体的1H- 15N HSQC谱图：样品初始状态（蓝色），25 ℃孵育8 h后的样品（红色）；(b) ATAD2 C1057A/C1079A/C1101A突变体蛋白的1H- 15N HSQC谱图：蛋白初始状态（蓝色），25 ℃孵育8 h后的蛋白（红色）；(c) ATAD2溴结构域野生型、ATAD2 C1101A突变体和ATAD2 C1057A/C1079A/C1101A突变体的30个NMR信号平均谱峰强度随时间的变化；(d) ATAD2溴结构域野生型（红色）与ATAD2 C1057A/C1079A/C1101A突变体（蓝色）的初始1H- 15N HSQC谱图叠加 |
| Fig. 6 (a) 1H- 15N HSQC spectra of C1101A mutant: The initial sample state (blue), the sample incubated at 25 ℃ for 8 h (red); (b) 1H- 15N HSQC spectra of C1057A/C1079A/C1101A mutant: The initial protein sample (blue), the sample incubated at 25 ℃ for 8 h (red); (c) The average signal intensities of 30 NMR resonances of wild-type ATAD2 bromodomain, C1101A mutant and C1057A/C1079A/C1101A mutant over time; (d) The superposition of the initial 1H-15N HSQC spectra of ATAD2 bromodomain wild-type (red) and C1057A/C1079A/C1101A mutant (blue) |
|
|