溶液中ATAD2溴结构域聚集行为的研究

王远方,王小花,舒畅,张许,刘买利,曾丹云

The Aggregation of ATAD2 Bromodomain in Solution

WANG Yuanfang,WANG Xiaohua,SHU Chang,ZHANG Xu,LIU Maili,ZENG Danyun

图2 (a) ATAD2溴结构域形成沉淀，左管为ATAD2溴结构域溶液，右管为缓冲液对照；(b)非变性PAGE检测ATAD2溴结构域聚集情况；图中Marker为蛋白Marker，1~5泳道分别是浓度为0.025 mmol/L、0.05 mmol/L、0.1 mmol/L、0.25 mmol/L、0.5 mmol/L的ATAD2溴结构域

Fig. 2 (a) The ATAD2 bromodomain formed gelatinous precipitates. The left tube contains ATAD2 bromodomain solution; the right tube contains buffer solution as a control. (b) The aggregation of ATAD2 bromodomain detected by native-PAGE: Swimlanes 1~5 correspond to 0.025 mmol/L, 0.05 mmol/L, 0.1 mmol/L, 0.25 mmol/L and 0.5 mmol/L of ATAD2 bromodomain in the loading samples, respectively