顺磁核磁共振技术研究蛋白质遭遇复合物的动态结构
Investigation of Dynamic Structure of Protein Encountering Complex with Paramagnetic NMR
顺磁核磁共振技术研究蛋白质遭遇复合物的动态结构 |
| 赵昶,龚洲 |
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Investigation of Dynamic Structure of Protein Encountering Complex with Paramagnetic NMR |
| ZHAO Chang,GONG Zhou |
| 图2 25 ℃下，EIIAGlc/HPr溶液体系（黑色）与EIIAGlc/HPr E5C (a)、EIIAGlc/HPr E66C (b)溶液体系（红色）的1H-15N HSQC谱图比较；EIIAGlc与HPr E5C (c)、EIIAGlc与HPr E66C (d)的分子间PRE实验观测值（红色圆点）和理论计算值（黑色实线）；(e) HPr探针连接位点结构图；(f) EIIAGlc的结构示意图，其中PRE实验值与理论计算值差异较大的区域用红色表示 |
| Fig. 2 1H-15N HSQC spectra of EIIAGlc/HPr solution (black) and EIIAGlc/HPr E5C (a) or EIIAGlc/HPr E66C (b) (red) were compared under 25 ℃. Experimental observations (red dots) and theoretical calculations (black solid lines) of intermolecular PRE of EIIAGlc and HPr E5C (c) or HPr E66C (d); (e) Structure diagram of HPr probe connection site; (f) Schematic diagram of EIIAGlc structure, where areas with large differences between PRE experimental values and theoretical calculated values are shown in red |
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