基于磁共振的胞浆中无标记酵母细胞色素c构象变化追踪
Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR
基于磁共振的胞浆中无标记酵母细胞色素c构象变化追踪 |
| 占建华,胡琴,朱勤俊,蒋滨,张许,刘买利 |
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Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR |
| ZHAN Jianhua,HU Qin,ZHU Qinjun,JIANG Bin,ZHANG Xu,LIU Maili |
| 图2 不同构象cyt c的UV-Vis光谱和NMR谱. (a)经SLA处理过的cyt c、(b)经K3[Fe(CN)6]处理过的cyt c、(c)分别经SLA和K3[Fe(CN)6]处理过的cyt c等比例混合、(d)经H2O2处理过的cyt c的UV-Vis光谱；(e)经SLA处理过的cyt c、(f)经K3[Fe(CN)6]处理过的cyt c、(g)分别经SLA和K3[Fe(CN)6]处理过的cyt c等比例混合、(h)经H2O2处理过的cyt c的2D 1H-13C HSQC谱图 |
| Fig. 2 UV-Vis absorption spectra and NMR spectra of cyt c in different conformations. UV-Vis spectra of (a) cyt c treated with SLA, (b) cyt c treated with K3[Fe(CN)6], (c) cyt c mixed in equal proportions treated with SLA and K3[Fe(CN)6], respectively, (d) cyt c treated with H2O2; 2D 1H-13C HSQC spectra of (e) cyt c treated with SLA, (f) cyt c treated with K3[Fe(CN)6], (g) cyt c mixed in equal proportions treated with SLA and K3[Fe(CN)6], respectively, (h) cyt c treated with H2O2 |
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