蛋白质二硫键异构酶与α-突触核蛋白的相互作用及对其聚集的影响
Inhibition of α-Synuclein Aggregation by the Interaction Between Protein Disulfide Isomerase and α-Synuclein
蛋白质二硫键异构酶与α-突触核蛋白的相互作用及对其聚集的影响 |
| 裴云山,张偲,刘晓黎,成凯,张则婷,李从刚 |
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Inhibition of α-Synuclein Aggregation by the Interaction Between Protein Disulfide Isomerase and α-Synuclein |
| Yun-shan PEI,Cai ZHANG,Xiao-li LIU,Kai CHENG,Ze-ting ZHANG,Cong-gang LI |
| 图2 ThT荧光实验检测PDI、PDI ab及PDI b'xa'对αsyn聚集的影响. (a) αsyn不加（黑色）和加入等量PDI ab（粉色）、PDI b'xa'（蓝色）、PDI（红色）后的纤维生长动力学曲线；(b)由动力曲线拟合得到的纤维生长达半数平台期所需时间t1/2及其统计分析，ns表示无明显差异，****表示p < 0.0001；圆点表示四组平行实验所求得的数值 |
| Fig.2 The effect on αsyn aggregation of PDI, PDI ab and PDI b'xa' detected by ThT experiment. (a) Aggregations kinetics of αsyn in the absence (black) and presence of equimolar PDI ab (pink), PDI b'xa' (blue) and PDI (red). (b) The fitted half-time (t1/2) values of aggregation and their statistical analysis. ns: not significant, ****: p < 0.0001; the dots represent the values obtained from four parallel experiments |
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