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T69E模拟磷酸化修饰对Bcl-2与Nur77相互作用的影响
徐倩1,2,陈朗1,2,胡翔颖1,李从刚1,刘乙祥1,*(),姜凌1,*()
The Effect of T69E-mimicked Phosphorylation on the Interaction Between Bcl-2 and Nur77
Qian XU1,2,Lang CHEN1,2,Xiang-ying HU1,Cong-gang LI1,Yi-xiang LIU1,*(),Ling JIANG1,*()

图2. 野生型胞内全长Bcl-2（wtBcl-2）蛋白的纯化和验证.(a)免疫印迹法检测wtBcl-2蛋白的表达纯化；(b)圆二色谱检测wtBcl-2蛋白的二级结构；(c) 15N标记的0.2 mmol/L wtBcl-2的15N-1H TROSY谱图

Fig.2. The purification and validation of wild type full-length Bcl-2 (wtBcl-2). (a) The purified wtBcl-2 validated by Western blot; (b) The secondary structure of the purified wtBcl-2 detected by using CD spectrum; (c) 15N-1H TROSY spectrum of 15N-labeled 0.2 mmol/L wtBcl-2