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基于19F化学标记磷酸化泛素的温度传感器开发
张志武1,2,杨菊1,2,聂泽锋1,2,叶尚祥1,3,*(),董旭1,*(),唐淳1,3,4
Development of a Temperature Senor Based on 19F-labeled Phosphorylated Ubiquitin
Zhi-wu ZHANG1,2,Ju YANG1,2,Ze-feng NIE1,2,Shang-xiang YE1,3,*(),Xu DONG1,*(),Chun TANG1,3,4

图5. (a) 不同温度下19F-K48C pUb的19F NMR谱图;(b)两种构象的比例变化和温度梯度之间呈线性

Fig.5. (a) The 19F NMR spectra of 19F-K48C pUb at different temperature; (b) The linear relationship between the ratio change of the two conformations and the temperature gradient