基于19F化学标记磷酸化泛素的温度传感器开发
张志武1,2,杨菊1,2,聂泽锋1,2,叶尚祥1,3,*(
),董旭1,*(),唐淳1,3,4
),董旭1,*(),唐淳1,3,4
Development of a Temperature Senor Based on 19F-labeled Phosphorylated Ubiquitin
Zhi-wu ZHANG1,2,Ju YANG1,2,Ze-feng NIE1,2,Shang-xiang YE1,3,*(),Xu DONG1,*(),Chun TANG1,3,4
),Xu DONG1,*(),Chun TANG1,3,4
图1. 15N-pUb样品在(a) 10 ℃和(b) 25℃温度下的1H-15N HSQC谱图：当温度升高，舒展态（缩写为rl）的信号强度降低，收缩态（缩写为rt）的信号强度升高
Fig.1. 1H-15N HSQC spectra of 15N-pUb sample at (a) 10 ℃ and (b) 25 ℃: When the temperature increases, the signal intensity of the relaxed state (rl) decreases, while the signal intensity of the retracted state (rt) increases
