Alpha-突触核蛋白与完整线粒体相互作用的NMR研究
余锦波1,2,张偲1,2,张则婷1,徐国华1,*(
),李从刚1
),李从刚1
Interactions Between α-synuclein and Intact Mitochondria Studied by NMR
Jin-bo YU1,2,Cai ZHANG1,2,Ze-ting ZHANG1,Guo-hua XU1,*(),Cong-gang LI1
),Cong-gang LI1
图4. α-synuclein-PMSF线粒体样品的(a) SDS-PAGE和(b)蛋白免疫印迹检测结果. 图中Control表示α-synuclein的标准品对照，Sample表示α-synuclein-PMSF线粒体样品，Supernatant表示α-synuclein-PMSF线粒体样品离心后的上清，Precipitate表示α-synuclein-PMSF线粒体样品离心后的沉淀
Fig.4. (a) SDS-PAGE and (b) Western-blotting analysis of α-synuclein with PMSF in the presence of intact mitochondria (a) SDS-PAGE analysis showing integrity of mitochondria; (b) Western-blotting analysis showing that α-synuclein is undegraded; Control: α-synuclein standard sample, Sample: α-synuclein with PMSF in the presence of intact mitochondria, Supernatant: the supernatant of the sample (α-synuclein with PMSF in the presence of intact mitochondria) that was centrifuged, Precipitate: the precipitate of the sample that was centrifuged
