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Alpha-突触核蛋白与完整线粒体相互作用的NMR研究
余锦波1,2,张偲1,2,张则婷1,徐国华1,*(),李从刚1
Interactions Between α-synuclein and Intact Mitochondria Studied by NMR
Jin-bo YU1,2,Cai ZHANG1,2,Ze-ting ZHANG1,Guo-hua XU1,*(),Cong-gang LI1

图2. 不同条件下α-synuclein的1H-15N SOFAST-HMQC谱图对比. (a) α-synuclein的氨基酸序列,组成N端的第1~60的残基用红色表示;(b) α-synuclein稀溶液(蓝色)与α-synuclein线粒体样品(红色)的谱图叠加;(c)含有(绿色)与不含有(蓝色)1 mmol/L PMSF的α-synuclein稀溶液的谱图叠加;(d) α-synuclein线粒体样品(红色)与α-synuclein-PMSF线粒体样品(青色)的谱图叠加

Fig.2. 1H-15N SOFAST-HMQC spectra of α-synuclein under different conditions. (a) The amino acid sequence of α-synuclein, the N-terminus region (1~60) are colored in red; (b) Spectral overlay of α-synuclein in absence (blue) and presence (red) of intact mitochondria, the partial peaks of which signal intensity attenuated in the presence of intact mitochondria are marked; (c) Spectral overlay of α-synuclein with (green) and without (blue) PMSF(1 mmol/L) in diluted solution; (d) Spectral overlay of α-synuclein with (cyan) and without (red) PMSF (1 mmol/L) in the presence of intact mitochondria