Alpha-突触核蛋白与完整线粒体相互作用的NMR研究
余锦波1,2,张偲1,2,张则婷1,徐国华1,*(
),李从刚1
),李从刚1
Interactions Between α-synuclein and Intact Mitochondria Studied by NMR
Jin-bo YU1,2,Cai ZHANG1,2,Ze-ting ZHANG1,Guo-hua XU1,*(),Cong-gang LI1
),Cong-gang LI1
图1. 分离提取的大鼠肝脏线粒体纯度和活力的表征. (a)线粒体特征蛋白COX6c和(b)胞质特征蛋白SOD1的蛋白免疫印迹检测结果，最右孔道为SOD1标样作为阳性对照，用以表征线粒体的纯度，Mito表示线粒体；(c)~(e)荧光共聚焦表征线粒体的活性，(c)为明场图，(d)为荧光共聚焦图，(e)为明场与荧光共聚焦叠加图
Fig.1. The analysis of isolated mitochondrial purity and viability from the liver of rat. (a) The COX6c component and (b) SOD1 component were detected by western blotting (SOD1 standard sample as positive control in figure (b)), showing the mitochondrial purity. (c)~(e) Mitochondrial viability characterized by fluorescence confocal microscopy: (c) Bright field; (d) Isolated mitochondria were stained with Mito Tracker® Red CMXRos whose accumulation is dependent upon the mitochondrial membrane potential (MMP); (e) Overlay of figure (c) and figure (d)
