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Interaction of GB1 with Metal Ions Studied by NMR Spectroscopy
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CHENG Kai, YAO Chen-die, XU Guo-hua, LI Cong-gang
Chinese Journal of Magnetic Resonance, 2018, 35(1): 1-7.
DOI: 10.11938/cjmr20172575
B1 domain of staphylococcal protein G (GB1) is a widely used model protein for developing in vivo and in vitro protein structural determination methods based on paramagnetic nuclear magnetic resonance (NMR) such as pseudocontact chemical shift (PCS ) and paramagnetic relaxation enhancement (PRE ). However, few previous studies have investigated the interactions between GB1 and metal ions, especially paramagnetic ions. In this study, the interactions between GB1 and divalent/lanthanide metal ions were studied by NMR spectroscopy. It was found that GB1 weakly bound with paramagnetic lanthanide ions and paramagnetic divalent ions, including Cu2+ , Mn2+ and Co2+ . In contrast, GB1 did not bind with diamagnetic divalent ions, such as Ca2+ , Mg2+ and Zn2+ . Furthermore, it was demonstrated that there were two binding sites for Cu2+ in GB1, but only one for lanthanide ions and divalent ions Mn2+ and Co2+ . The current study demonstrated that NMR spectroscopy is a powerful tool to study weak binding between protein and metal ions. And the results indicated that care must be taken to avoid possible interference to paramagnetic NMR data when using GB1 as the model protein.
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NMR-Based Metabonomic Analyses on Spleen Tissues of 4T1 Tumor-Bearing Mice Subjected to Chemotherapies with Different Drug Delivery Strategies
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HU Yi-li, HAO Fu-hua, WANG Yu-lan
Chinese Journal of Magnetic Resonance, 2018, 35(1): 8-21.
DOI: 10.11938/cjmr20172576
The combination of doxorubicin and paclitaxel is widely used in clinical cancer therapy. Due to enhanced permeation and retention effects of nanomaterials in tumor tissues, nanocarriers with good biocompatibility have been designed to encapsulate doxorubicin/paclitaxel, and used to facilitate delivery of the drugs to achieve better therapeutic outcome. In this study, NMR-based metabonomic analyses were used to study the metabolic profiles in spleen tissues of normal healthy mice and of 4T1 tumor-bearing mice, which were treated with either saline, or doxorubicin/paclitaxel in their free forms, or doxorubicin/paclitaxel encapsulated in nanocarriers. The results demonstrated that treatment with the free forms of doxorubicin/paclitaxel remitted tumor-induced disturbances in gut microbiota, while treatment with nanocarrier-encapsulated doxorubicin/paclitaxel alleviated increased glucose and fumarate metabolism associated with tumor bearing. In contrast, tumor-related splenomegaly and metabolic alterations in amino acids, organic acids, nucleic acids and choline, both attributable to immune responses of the spleen, were not ameliorated by drug treatment. This could also be due to the fact that the treatment period was too short to have any significant effects. This work provided metabolic phenotypical information of the spleen tissues in 4T1 tumor-bearing mice, and helped to understand the metabolic impacts of drug delivery systems on the spleen.
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Correlations Between Morphological Characteristics and Expression Levels of Specific Molecular Biomarkers in Glioblastoma
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MA Yun, GUO Hong, WANG Qiu-shi, ZHANG Wei-guo, WU Hao
Chinese Journal of Magnetic Resonance, 2018, 35(1): 22-30.
DOI: 10.11938/cjmr20172560
To provide guidance to clinical diagnosis and develop individual-based treatment/prognostic evaluation, this study explored the correlations between morphological characteristics of glioblastoma (GBM) measured by conventional MRI and expression levels of GBM-specific molecular biomarkers. MRI data of 60 patients with histopathologically confirmed GBM were analyzed retrospectively, from which the morphological characteristics of GMB (i.e., TLD , ELD and CTE /TLD ratio) were measured. Histochemical staining was used to measure the levels of GBM-specific molecular biomarkers (i.e., EGFR, IDH-1, TP53, PTEN and NEFL), which were calculated as the product of staining index and stained area. Correlations among the morphological characteristics and the levels of molecular biomarkers were assessed using multivariate logistic regression analyses. There was a significant correlation between TLD and EGFR level (r =0.796). The correlation between TLD and PTEN level was positive (r =0.533), while the correlation between TLD and IDH-1 level was negative (r =-0.672). CTE /TLD had a positive correlation with EGFR (r =0.622) and PTEN (r =0.638) levels, along with a negative correlation with IDH-1 level (r =-0.493). ELD had no significant correlation with the levels of all five molecular biomarkers. It was concluded that the morphological characteristics of GBM derived from conventional MRI had correlations with the expression levels of EGFR, IDH-1 and PTEN, and it was possible to derive heterogeneity, molecular classification and prognosis of GBM using conventional MRI.
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A New Combination Scheme of GRAPPA and Compressed Sensing for Accelerated Magnetic Resonance Imaging
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HUANG Li-jie, SONG Yang, ZHAO Xian-ce, XIE Hai-bin, WU Dong-mei, YANG Guang
Chinese Journal of Magnetic Resonance, 2018, 35(1): 31-39.
DOI: 10.11938/cjmr20172578
Both compressed sensing (CS) and parallel imaging (PI) can be used to accelerate magnetic resonance imaging (MRI) by under-sampling the k space data. Several methods combining CS and PI have been proposed to further improve the scanning speed. In this paper, we proposed a new approach to combine CS and PI. We used GRAPPA (Generalized Autocalibrating Partially Parallel Acquisitions) algorithm to reconstruct local under-sampled k space data, and CS to reconstruct the whole k space data for each coil. In the CS reconstruction step, we constrained that the reconstructed k space data should be assimilated to both the sampled k space data and the reconstructed k space data by GRAPPA. In addition, we designed a new sampling strategy to improve the quality of image reconstruction. In vivo imaging results demonstrated that the proposed approach could effectively remove artifacts and improve the image quality.
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Design and Implementation of a Novel Processing Pipeline for Magnetic Resonance Images
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LUO Qing, ZHANG Cheng-xiu, LI Wen-jing, ZHENG Hui, XIE Hai-bin, YANG Guang
Chinese Journal of Magnetic Resonance, 2018, 35(1): 40-51.
DOI: 10.11938/cjmr20172588
Reconstruction, post-processing and visualization of images are important parts of magnetic resonance imaging (MRI). In this work, a new open source framework, namely Yet Another Pipeline (YAP), was proposed for reconstruction, post-processing and visualization of MR images. With YAP, researchers can build customized image-processing pipelines with ease. Compared with the existing open source frameworks, YAP has the following features:(1) it has an interface-based design, enabling function extension by plugin development; (2) it uses a script language with straightforward grammar, making it easy to build and modify customized pipelines; (3) it supports branches in the pipelines, facilitating pipeline building and debugging. YAP has been implemented on a commercial MRI system.
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A Method for Eddy Current Field Measurement in Permanent Magnet Magnetic Resonance Imaging Systems
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SONG Rui, HE Yan-fa, ZHANG Bo
Chinese Journal of Magnetic Resonance, 2018, 35(1): 52-59.
DOI: 10.11938/cjmr20172593
Magnetic resonance imaging (MRI) is a widely used medical imaging technique. In MRI system, gradient magnetic fields are used to code spatial information. However, the fast-switching electric currents in the gradients coils used to generate gradient fields also induce vortex electric field, often referred as eddy current, in the surrounding metal conductors. In this paper, a method for eddy current field measurement was proposed. Based on the Faraday law of electromagnetic induction, an eddy current field measuring device was designed. Combining hardware acquisition and software processing, the eddy current field was obtained by subtracting the ideal gradient field from the magnetic field measured experimentally, whose waveform could be displayed in real time. The proposed method was verified by experimental results.
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Measurement of Crosslinking Density of Rubbers by Analyzing Gaussian-Weighted 1 H CPMG Relaxation Curves
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GAO Peng-fei, CHU Lin-lin, YANG Yi, JIANG Ji-feng, WANG Jia-chen, YAO Ye-feng, ZHOU Bing
Chinese Journal of Magnetic Resonance, 2018, 35(1): 60-74.
DOI: 10.11938/cjmr20172590
CPMG sequence is widely used to measure crosslink density of rubber. However, the crosslink density obtained by this technique has a strong dependence on the interval times between the pulses in CPMG sequence, and the results measured have only a weak correlation with rubber hardness. To overcome this shortcoming, we proposed in this work to construct a 1 H T 2 relaxation curve by summing Gaussian-weighted CPMG relaxation curves acquired at by varying the interval time between the pulses in CPMG sequence. Using the multi-echo Gaussian-weighted relaxation curve to calculate the crosslink density gave results that had greatly improved correlation with the rubber hardness. The new method was also fast and efficient, as it took only one-tenth of the experimental time required for the conventional 1 H double quantum NMR (1 H DQ NMR) experiment.
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An NMR Study on How Steric Effects Affect Synergistic Effects in Surfactant Mixtures
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CHEN Xiao-ying, YU Gang-jin, MAO Shi-zhen, LIU Mai-li, DU You-ru
Chinese Journal of Magnetic Resonance, 2018, 35(1): 75-80.
DOI: 10.11938/cjmr20172577
NMR spectra of aqueous solutions of cetyltrimethylammonium bromide (CTAB)/triton X-100 (TX-100), sodium dodecyl sulfate (SDS)/TX-100 and CTAB/TX-100/SDS surfactant mixtures were acquired. The proton chemical shifts of each component and their dependence on concentration were determined and used to derive the critical micelle concentrations (CMC s) of CTAB, TX-100 and SDS in each mixture. It was found that, in several cases, the role played by the steric effect in the synergism of surfactant mixtures were not less than that of the electrostatic interaction. The larger the cross sectional area of the polar head was, the greater the synergy would be. It is concluded that the steric effect is a major player in the synergistic effect of surfactant mixtures, especially when the electrostatic interaction is weak.
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An NMR Study on Two Mixed Micelles Formed by Nonionic Surfactants
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ZHAO Rui-ge, ZHANG Wei-hong, MA Er-qian, YU Hai-tao, YANG Qiu-qing
Chinese Journal of Magnetic Resonance, 2018, 35(1): 81-89.
DOI: 10.11938/cjmr20172587
NMR techniques were used to study the relative arrangement and interaction sites in mixed micelles formed in NP-10/Tween40 and NP-10/C10 E6 aqueous solutions. The results of two-dimensional NOESY experiments and relaxation time measurements showed that the interaction site of these two pairs of mixed surfactants were similar, but the intermolecular interaction between NP-10 and Tween40 was stronger than that between NP-10 and C10 E6 . T 2 measurements demonstrated that inter-heteromolecular interactions decreased when the molar concentrations of Tween40 and C10 E6 were larger than 2 and 4 folds of that of NP-10, respectively. Under such circumstances, the inter-homomolecular interactions began to play a key role, indicative of the ideal mixture molar ratio and the change of synergistic effect in the complex systems. T 2 /T 1 ratio measurements indicated that molecular chain movement in the NP-10/Tween40 mixture was more limited than that in the NP-10/C10 E6 mixture. The above conclusions were verified by spatial distance measurements.
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Structural Elucidation and NMR Spectral Assignments of Two C19 -Diterpenoid Alkaloids
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YIN Tian-peng, CHEN Yang, LUO Ping, CAL Le, DING Zhong-tao
Chinese Journal of Magnetic Resonance, 2018, 35(1): 90-97.
DOI: 10.11938/cjmr20172583
Two C19 -diterpenoid alkaloids, homochasmanine (compound 1 ) and 14-debenzoylfranchetine (compound 2 ), were isolated from the roots of Aconitum handelianum . Their structures were elucidated by one-dimensional and two-dimensional nuclear magnetic resonance (NMR) techniques, including 1 H NMR, 13 C NMR, DEPT, 1 H-1 H COSY, HSQC, HMBC and NOESY. The 1 H and 13 C NMR signals of the compounds were completely assigned.
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Spectral Analyses of a Novel Ibuprofen-Phillygenin Ester
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FAN Hong-yu
Chinese Journal of Magnetic Resonance, 2018, 35(1): 98-108.
DOI: 10.11938/cjmr20172584
An ibuprofen-phillygenin ester was synthesized through the Schotten-Baumann reaction with ibuprofen and phillygenin as the starting materials. Ultraviolet absorption (UV) spectrum, infrared absorption (IR) spectrum, matrix-assisted laser desorption/ionization-mass spectra (MALDI-TOF-MS), and one-dimensional/two dimensional nuclear magnetic resonance (NMR) spectra (i.e., 1 H-NMR, 13 C-NMR, 13 C-DEPT, 1 H-1 H COSY, 1 H-1 H NOESY, 1 H-13 C HSQC and 1 H-13 C HMBC) of the compound were collected. All 1 H and 13 C NMR signals were assigned.
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Spectral Analyses of Ecabet Sodium
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JI Xiao-long, YU Shui-tao, CHEN Zai-xin
Chinese Journal of Magnetic Resonance, 2018, 35(1): 109-118.
DOI: 10.11938/cjmr20172591
Ultraviolet (UV) spectrum, infrared (IR) spectrum, high resolution-mass spectrum (HR-MS), nuclear magnetic resonance (NMR) spectra (i.e., 1 H NMR, 13 C NMR, DEPT, 1 H-1 H COSY, 1 H-13 C HSQC and 1 H-13 C HMBC) of ecabet sodium were collected and analyzed to elucidate its structure. All 1 H and 13 C NMR signals were assigned.
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An NMR Study on Prucalopride
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ZHOU Zhong-gao, YUAN Yang-yang, LIU Hong-bo, QI Qi, XIE Yong-rong
Chinese Journal of Magnetic Resonance, 2018, 35(1): 119-127.
DOI: 10.11938/cjmr20172592
4-Amino-5-chloro-2,3-dihydro-N-[1-(3-methoxypropyl)-4-piperidinyl]-7-benzofurancarboxamide was synthesized by amide condensation reaction using 4-amino-5-chloro-2,3-dihydrobenzofuran-7-carboxylic acid and 1-(3-methoxypropyl)-4-piperidinamine as the reactants. The resultant compound, also named prucalopride, is a derivative of dihydrobenzofuran 5-H4 receptor antagonist, and an important drug for treating chronic constipation. The NMR spectrum of prucalopride is crowded and complex due to the fact that there are many carbon atoms in the molecule have similar chemical environment. In this study, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and liquid chromatographyhigh resolution mass spectrometry (LC-HRMS) were used to analyze the composition of prucalopride. 1 H NMR, 13 C NMR, DEPT, 1 H-1 H COSY, 1 H-13 C HSQC and 1 H-13 C HMBC spectra of the compound were collected, and its 1 H and 13 C NMR signals were assigned.