Dimer Form of Archaeal Protein SSO6904 Identified by NMR

Abstract

Abstract:

Archaeal protein SSO6904 is a newly-discovered protein with weak calcium-binding activity. Protein SSO6904 is an all-helices protein. Protein purification by gel filtration discovered both monomer and dimer forms of SSO6904. ¹H NMR spectra indicated that the monomer and dimer have similar structural core. Analysis of 2D ¹H-¹⁵N HSQC spectra revealed that the major structural difference between the monomer and dimer was at the two loops connecting helices 2 and 3, and helices 5 and 6, respectively. These two loops form a key structural region for constructing the dimer, while other regions of monomer and dimer have the same structures. Structural analysis proposed a domain-swapped model of the SSO6904 dimer. The domain-swapped dimer may regulate the stability and function of SSO6904.

Key words: NMR, protein structure, HSQC, SSO6904, archaea, protein dimer

CLC Number:

YAO Hong-Wei, FENG Yin-Gang, WANG Jin-Feng. Dimer Form of Archaeal Protein SSO6904 Identified by NMR[J]. Chinese Journal of Magnetic Resonance, 2012, 29(2): 224-230.

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