A Method for Improving the Resolution of Two-Dimensional NMR Spectrum by Overlapping HSQC and 1H, 13C-COSY

Chinese Journal of Magnetic Resonance

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A Method for Improving the Resolution of Two-Dimensional NMR Spectrum by Overlapping HSQC and ¹H, ¹³C-COSY

ZHU Chuan-jun^1,3，CUI Yu-xin⁴，JIANG Biao^1,2*

1. Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China; 
2. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; 
3. Shanghai Torch Innovation Park of Fine Chemical Industry, Analysis Center, Shanghai 201512, China; 
4. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China

Online:2013-03-05 Published:2013-03-05
Contact: Jiang Biao, Tel: 021-20325008，E-mail: jiangb@mail.sioc.ac.cn.
About author:朱传钧（1984-），男，北京人，硕士，助理研究员，主要从事核磁共振小分子结构分析研究.
Supported by:
国家自然科学基金（重点项目）资助项目（20832007）；国家科技重大专项(重大新药创制)资助项目（2009ZX09501-016，2009ZX09501-007）.

Abstract

Abstract:

In traditional two-dimensional (2D) NMR experiments, spectral resolution in the F₁ dimension is usually higher than that in the F₂ dimension. Therefore, in the HSQC experiments, the spectral resolution in the ¹³C dimension is lower than that in the ¹H dimension. This is the opposite in the ¹H, ¹³C-COSY experiments. In this work, a method for improving the resolution of 2D NMR spectrum was presented, in which HSQC and ¹H, ¹³C-COSY spectra were overlapped, and then the weaker points were selected, saved and displayed. The method was implemented as an AU program within the Bruker Topspin framework. It was demonstrated experimentally that the method could improve spectral resolution, at no cost of signal-to-noise ratio. The method can also be used to suppress F₁ noise.

Key words: NMR, resolution, AU program, overlap, HSQC, ¹H, ¹³C-COSY

CLC Number:

O482.53

ZHU Chuan-jun，CUI Yu-xin，JIANG Biao*. A Method for Improving the Resolution of Two-Dimensional NMR Spectrum by Overlapping HSQC and ¹H, ¹³C-COSY[J]. Chinese Journal of Magnetic Resonance.

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A Method for Improving the Resolution of Two-Dimensional NMR Spectrum by Overlapping HSQC and ¹H, ¹³C-COSY

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