Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR

doi:10.11938/cjmr20222985

Abstract

Abstract:

Cytochrome c (cyt c) is an important multifunctional protein. In mitochondria, it acts as a carrier for electron transporting. In cytoplasm, it may act as an apoptotic initiator to initiate the apoptotic process. Whether and how a complex cytoplasmic environment affects its conformation has not been confirmed yet. In this study, the conformational changes of wild type saccharomyces cerevisiae iso-1 cyt c in yeast cell homogenate were tracked by methyl-based nuclear magnetic resonance (NMR) technique. At least four different oxidative conformations and one reduced conformation of cyt c were identified in cell homogenate. And over time, the transitions among different conformations of cyt c were observed. The results indicate that the conformation of cyt c changes with environment, which may be closely related to resistance to oxidative stress.

Key words: nuclear magnetic resonance (NMR), conformational change, methyl, cytochrome c

CLC Number:

O482.53

ZHAN Jianhua,HU Qin,ZHU Qinjun,JIANG Bin,ZHANG Xu,LIU Maili. Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR[J]. Chinese Journal of Magnetic Resonance, 2023, 40(1): 22-29.

Figures/Tables 4

References 29

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