Chinese Journal of Magnetic Resonance ›› 2017, Vol. 34 ›› Issue (4): 453-464.doi: 10.11938/cjmr20172580

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NMR Fingerprints of Eucommia ulmoides Oliver

JIANG Yang-ming1,2, ZOU Yun-yun1,2, HUANG Tao1,2, CHEN Yan1,2, PAN Wei-dong1,2, HAO Fu-hua3, Zhang Jian-xin1,2   

  1. 1. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China;
    2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China;
    3. Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China
  • Received:2017-05-12 Revised:2017-11-01 Online:2017-12-05 Published:2017-11-29

Abstract: The 1H NMR fingerprint of Eucommia ulmoides acquired with a CPMG pulse sequence was analyzed with the complete reduction to amplitude-frequency table (CRAFT) approach. The signals of target compounds were extracted without chemical separation and purification. Quantitative analysis showed that the average concentration of pinoresinol glucoside (PDG) in Eucommia ulmoides got from Guiyang medical plant garden was 0.275 6% with a relative standard deviation (RSD) of 1.69%. The results were consistent with those obtained by high performance liquid chromatography (average content of 0.269 6% and RSD of 0.65%). NMR fingerprint and multivariate statistical analysis also revealed that there existed significant differences between Hunan and Guizhou Eucommia ulmoides Oliver.

Key words: NMR fingerprint, complete reduction to amplitude-frequency table (CRAFT), Eucommia ulmoides Oliver, quantitative/quanlitative analysis, pinoresinol glucoside (PDG)

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