Effects of Metal Ions on Human Serum Albumin Studied by Radiation Damping Water-Ligand Observed via Gradient Spectroscopy

doi:10.11938/cjmr20162527

Abstract

Abstract: Human serum albumin (HSA) contains many metabolite binding sites. It has been widely studied for its functions in drug transportation. Nuclear magnetic resonance (NMR) is a frequently used tool to study HSA. However, due to its high molecular weight, the NMR spectra of HSA acquired with the conventional methods are often crowded, making spectral assignment and data interpretation difficult. In this study, a new method, which combined radiation damping water-ligand observed via gradient spectroscopy (RD-WaterLOGSY) with transverse relaxation weighted (T₂W) techniques, was proposed to simplify the NMR spectra of HSA. With the T₂W-RD-WaterLOGSY technique, the effect of pH on HSA was studied, as well as the interactions between HSA and Zn²⁺. The results showed that the relative changes in chemical shift could be used as a probe to analyze the pH changes in HSA solution and the interactions between HSA and Zn²⁺.

Key words: NMR, human serum albumin (HSA), Zn²⁺, interaction, T₂W-RD-WaterLOGSY

CLC Number:

CHEN Yao, SUN Peng, LIU Mai-li, ZHANG Xu. Effects of Metal Ions on Human Serum Albumin Studied by Radiation Damping Water-Ligand Observed via Gradient Spectroscopy[J]. Chinese Journal of Magnetic Resonance, 2017, 34(3): 266-274.

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