A Quantitative NMR Method to Analyze Vitamin B1, Vitamin B2, Nicotinamide and Calcium Pantothenate Contents in Compound Vitamin B Tablets

doi:10.11938/cjmr20160309

Abstract

Abstract:

A quantitative ¹H NMR method was established for analysis of contents of vitamin B1, vitamin B2, nicotinamide and calcium pantothenate in Compound Vitamin B Tablets. The experimental settings were as following: delay time (D₁) 1.0 s, pulse width (P₁) 3.0 μs, number of sampling (NS) 500 times, 1,4-benzenedialdehyde peak at δ 10.12 (2H) as the internal standard, and deuterated DMSO-d₆ as the solvent. The peaks for quantitative analysis were: vitamin B1 at δ 9.60~9.90 (1H), vitamin B2 at δ 11.36 (1H), nicotinamide at δ 9.01 (1H) and calcium pantothenate at δ 0.78 (12H). The slopes of the standard curves obtained for the 4 components were close to theoretically predicted values. Calibration with vitamin B1 and calcium pantothenate reference substances yielded correlation coefficients (r) above 0.999 and recovery ratio near 100%. Finally, the method was applied to 4 real samples, and the results suggested that it is simple to use, accurate and reliable.

Key words: quantitative ¹H NMR, Compound Vitamin B Tablets, vitamin B1 (VB1), vitamin B2 (VB2), nicotinamide, calcium pantothenate

CLC Number:

O482.53
R917

GUO Qiang-sheng, LIU Ming-ke, YU Shan, SHEN Ting-ting, SONG Wei, XU Xu. A Quantitative NMR Method to Analyze Vitamin B1, Vitamin B2, Nicotinamide and Calcium Pantothenate Contents in Compound Vitamin B Tablets[J]. Chinese Journal of Magnetic Resonance, 2016, 33(3): 442-451.

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