Chinese Journal of Magnetic Resonance

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Metabolite Composition of Planarian Analyzed by Nuclear Magnetic Resonance Spectroscopy

CHEN Lu1,2,WANG Yu-lan1*   

  1. 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences),  Wuhan 430071, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2013-04-08 Revised:2013-04-23 Online:2013-12-05 Published:2013-12-05
  • About author:*Corresponding author: Wang Yu-lan, Tel: 027-87197143, E-mail: yulan.wang@wipm.ac.cn.
  • Supported by:

    国家自然科学基金资助项目(21221064).

Abstract:

Planarian is a free-living worm belonging to the Phylum Platyhelminthes. Because of its special evolutionary status and remarkable regenerative abilities, planarian has emerged as an ideal model organism for the study of experimental zoology, cytogenetics, developmental biology and regenerative biology. Although significant advances have been made in recent studies of planarian, little information is available in metabolome of the worm. In this work, we prepared samples of planarian body extract with the aqueous methanol extraction method, and analyzed the samples with 1D 1H NMR spectroscopy and 2D NMR spectroscopy (e.g., 1H-1H COSY, 1H-1H TOCSY, 1H-1H J-RES, 1H-13C HSQC and 1H-13C HMBC) on a high-resolution 1 GHz NMR spectrometer. Fifty three metabolites were identified from the planarian body extracts, including amino acids, saccharides, lipids, organic acids, ketone bodies, amines, choline and nucleotide metabolites. The results of this study will provide a basic dataset for planarian metabolome research.

Key words: NMR, assignments, metabolome, planarian

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