NMR-Based Metabonomics Analysis of Biofluids and Tissues in Kunming Mice

Abstract

Abstract:

Kunming (KM) mouse is a widely used model animal species in China. We measured the metabolites in plasma, urine, liver and other tissues from KM mice with nuclear magnetic resonance (NMR)-based metabonomic analysis. The representative ¹H NMR spectra of biofluids and tissues from KM mice were assigned with the aid of a range of two-dimensional NMR techniques, including COSY (Correlation Spectroscopy), TOCSY (Total Correlation Spectroscopy), J-Res (J-Resolved Spectroscopy), HSQC (Heteronuclear Single Quantum Coherence) and HMBC (Heteronuclear Multiple Bond Coherence). A total of 82 different metabolites, including 46 metabolites from the urine, 32 from the plasma, 43 from the liver extracts and 39 from the kidney cortex extracts, were identified. It was found that some metabolites existed in all the samples. Our results provide a foundation for future metabonomic studies using KM mice as the model animal.

Key words: NMR, Kunming mice, biofluids, metabonomics

CLC Number:

O482.53

CAI Yi，HAO Fu-hua，WANG Yu-lan*. NMR-Based Metabonomics Analysis of Biofluids and Tissues in Kunming Mice[J]. Chinese Journal of Magnetic Resonance.

References

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NMR-Based Metabonomics Analysis of Biofluids and Tissues in Kunming Mice

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