19F NMR Techniques and Their Applications in Protein Studies

Abstract

Abstract:

¹⁹F nucleus has unique properties, such as that it is a 1/2 spin; natural abundance is 100%, having comparable sensitivity as ¹H; chemical shift dispersion is large and ultra sensitive to the environment; most biological samples do not contain ¹⁹F, such that there is no interference background signal, etc. These properties make ¹⁹F NMR an attractive approach to study biological systems. ¹⁹F NMR has wide applications in magnetic resonance imaging, pharmacology chemistry and studies of biomacromolecules. There are many reviews on these topics. Here we will focus on the applications of ¹⁹F NMR in protein studies, including ¹⁹F labeling methods, protein structure and dynamics characterization, protein folding, protein-drug interactions and in-cell protein NMR.

Key words: ¹⁹F NMR, protein structure, protein dynamics, drug recognition, in-cell NMR

CLC Number:

O482.53

WANG Gui-Fang, ZHANG Ze-Ting, XU Guo-Hua, LIU Xiao-Li, WU Qiong, BAI Jia, YE Yan-Sheng, LI Cong-Gang, LIU Mai-Li . ¹⁹F NMR Techniques and Their Applications in Protein Studies[J]. Chinese Journal of Magnetic Resonance, 2012, 29(4): 621-637.

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¹⁹F NMR Techniques and Their Applications in Protein Studies

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