一个获得压水峰良好效果的简单方法

波谱学杂志

一个获得压水峰良好效果的简单方法

毛希安*

波谱与原子分子物理国家重点实验室，武汉磁共振中心（中国科学院武汉物理与数学研究所），湖北武汉 430071

收稿日期:2013-10-08 修回日期:2013-11-07 出版日期:2014-03-05 发布日期:2014-03-05
作者简介:毛希安(1952-)，江西南城人，博士，研究员，电话：+01-216-368-7068, E-mail: xian.mao@case.edu. *通讯联系人．

A Simple Method for Clean Water Signal Suppression

MAO Xi-an*

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China

Received:2013-10-08 Revised:2013-11-07 Online:2014-03-05 Published:2014-03-05
About author:*Corresponding author：MAO Xi-an, Tel: +01-216-368-7068, E-mail: xian.mao@case.edu.

摘要/Abstract

摘要：

蛋白质NMR中最基本的实验是HSQC实验．在HSQC实验中，水峰压制的主要方法之一是利用一对与异核旋磁比比值相适应的梯度脉冲．该文介绍一种简单易行的方法，只需对这一对脉冲进行适当的延长便能使得水峰几乎完全消失.

关键词: HSQC实验, 水峰压制, 梯度脉冲, 延长散相时间

Abstract:

HSQC experiments are the most basic experiments in protein NMR. In HSQC experiments, water suppression is mainly achieved by a pair of field gradient pulses whose intensity ratio is in accordance with the ratio of the heteronuclear gyromagnetic ratios. In this paper, a simple method is propsed to achieve clean water suppression in HSQC experiments by properly modulating the gradient pulse length.

中图分类号:

O482.53

毛希安. 一个获得压水峰良好效果的简单方法[J]. 波谱学杂志.

MAO Xi-an. A Simple Method for Clean Water Signal Suppression[J]. Chinese Journal of Magnetic Resonance.