波谱学杂志 ›› 1995, Vol. 12 ›› Issue (6): 635-639.

• 研究论文 • 上一篇    下一篇

用快速低温冷冻和ESK方法分析兔脂肪栓塞脑组织中自由基

徐杰1, 刘桂珍1, 詹瑞云1, 王江2   

  1. 1. 中国科学院长春应用化学研究所应用谱学开放实验室, 长春 130022;
    2. 第一汽车集团公司医院, 长春 130011
  • 收稿日期:1994-11-01 出版日期:1995-12-05 发布日期:2018-01-17

THE ANALYSIS OF FREE RADICALS IN RABBIT BRAIN TISSUES DUE TO VENOUS FAT-EMBOLISM BY A RAPIDLY-FROZEN AND ESR TECHNIQUE

Xu jie1, Liu Guizhen1, Zhan Ruiyun1, wan jiang2   

  1. 1. Applied Spectroscopy Lab, Changchun Institute of Applied Chemistry, The Chinese Academy of Sciences, Changchun 130022;
    2. The First Automobile Gruop Corparation Hospital, Changchun 130011
  • Received:1994-11-01 Online:1995-12-05 Published:2018-01-17

摘要: 用快速低温冷冻-ESR实验方法研究兔脂肪栓塞模型脑组织自由基的变化,实验发现脂肪栓塞实验组自由基相对浓度是正常对照组的2-3倍。ESR检测到脂类过氧化自由基和以碳为中心的烷基类自由基的事实表明脂肪栓塞病变存在活性氧自由基作用过程。文中对快速冷冻法可行性进行评估。

关键词: 兔脑组织, 脂肪栓塞, 自由基, 快速冷冻, ESR

Abstract: A Rapidly-frozen technique and ESR method were used to directly measure free radicals generation in rabbit brain tissues due to venous fat-embolism.It had been found that ESR signal intensities of free radicals for the experimental fat-embolism group are 2-3 times higher than those for norinal contrast groups.The appearance of the lipid peroxyl radicals and the carbon-centered alkyl radicals provides an evidence that the fat-embolism pathologenesis may involve an active-oxygen radical action.In this paper the advantage of the rapidly-frozen method and its feasibility are elucidated.

Key words: Rabbit brain tissues, Fat-embolism pathologenesis, Free mdical, Rapidly-frozen, ESK