用二维NMR交换谱定量地研究氘代DMSO和氘代氯仿中小肽活泼质子的交换动力学

波谱学杂志 ›› 1999, Vol. 16 ›› Issue (1): 21-27.

用二维NMR交换谱定量地研究氘代DMSO和氘代氯仿中小肽活泼质子的交换动力学

杜琛, 毛希安

中国科学院武汉物理与数学研究所, 波谱与原子分子物理国家重点实验室, 武汉 430071

收稿日期:1998-11-02 修回日期:1998-12-01 出版日期:1999-02-05 发布日期:2018-01-11
作者简介:杜琛,女,1973年出生,硕士研究生
基金资助:
Project supported by the National Natural Science Foundation of China under the number of 29725307.

QUANTITATIVE STUDY OF EXCHANGE DYNAMICS OF MOBILE PROTONS OF A SMALL PEPTIDE IN DMSO-d₆ AND CDCl₃ USING 2D EXSY

Du Chen, Mao Xi'an

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan 430071

Received:1998-11-02 Revised:1998-12-01 Online:1999-02-05 Published:2018-01-11
Supported by:
Project supported by the National Natural Science Foundation of China under the number of 29725307.

摘要/Abstract

摘要： 用核磁共振二维交换谱方法研究了类肽化合物Cbz-Ser-Tyr-OC₆H₁₃中活泼质子的交换动力学,结果表明,在DMSO-d₆和CDCl₃溶液中,四种活泼质子与溶剂中残留水的交换快慢顺序为:Ser-OH > Tyr-OH > Ser-NH > Tyr-NH.在此基础上,对氨基酸中氨基所处的局部环境进行了讨论.

关键词: 二维交换谱, 小肽, 活泼质子, 交换动力学

Abstract: NMR two dimensional (2D) exchange spectroscopy (EXSY) is used to study the exchange dynamics of mobile protons in a small pseudopeptide Cbz-Ser-Tyr-OC₆H₁₃. It is shown by a quantitative study that the exchange rates for the four mobile protons in this molecule with water are in the order Ser-OH~Tyr-OH > Ser-NH > Tyr-NH, under the given experimental conditions with dimethylsulfoxide and chloroform as solvents. Local structures for the amino groups in different solutions are described.

Key words: NMR, Proton Exchange, Peptide, Organic Solvents

杜琛, 毛希安. 用二维NMR交换谱定量地研究氘代DMSO和氘代氯仿中小肽活泼质子的交换动力学[J]. 波谱学杂志, 1999, 16(1): 21-27.

Du Chen, Mao Xi'an. QUANTITATIVE STUDY OF EXCHANGE DYNAMICS OF MOBILE PROTONS OF A SMALL PEPTIDE IN DMSO-d₆ AND CDCl₃ USING 2D EXSY[J]. Chinese Journal of Magnetic Resonance, 1999, 16(1): 21-27.