氨基酸共聚物修饰的生物相容性MRI造影剂

doi:10.11938/cjmr20160413

波谱学杂志 ›› 2016, Vol. 33 ›› Issue (4): 635-645.doi: 10.11938/cjmr20160413

氨基酸共聚物修饰的生物相容性MRI造影剂

湛游洋^1,2, 薛蓉¹, 祝云龙^1,2, 李晓晶¹, 裴奉奎¹, 冯江华³

1. 中国科学院长春应用化学研究所, 吉林长春 130022;
2. 中国科学院大学, 北京 100049;
3. 厦门大学电子科学系, 福建厦门 361005

收稿日期:2016-02-29 修回日期:2016-10-24 出版日期:2016-12-05 发布日期:2016-12-05
通讯作者: 李晓晶,电话:0431-85262219,E-mail:xjli@ciac.ac.cn E-mail:xjli@ciac.ac.cn
作者简介:湛游洋(1990-),女,黑龙江哈尔滨人,博士研究生,物理化学专业
基金资助:
国家自然科学基金资助项目（21305134）.

A Biocompatible Gadolinium-Based Amino Acid Copolymer Contrast Agent for Magnetic Resonance Imaging

ZHAN You-yang^1,2, XUE Rong¹, ZHU Yun-long^1,2, LI Xiao-jing¹, PEI Feng-kui¹, FENG Jiang-hua³

1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. Department of Electronic Science, Xiamen University, Xiamen 361005, China

Received:2016-02-29 Revised:2016-10-24 Online:2016-12-05 Published:2016-12-05

摘要/Abstract

摘要： 通过天门冬氨酸（Asp）、异亮氨酸（Ile）热缩聚反应，制备了天门冬氨酸-异亮氨酸共聚物（AI），通过乙二胺（EDA）胺化，并与1，4，7，10-四氮杂环十二烷-1，4，7，10-四羧酸（DOTA）连接，再与钆离子（Gd³⁺）络合，合成了生物相容性大分子磁共振成像（MRI）造影剂——AI-EDA-DOTA-Gd．运用红外光谱（IR）、核磁共振（NMR）、电感耦合等离子谱（ICP）等方法对其进行结构表征，并通过弛豫性能、溶血性质、急性毒性及动物体内成像对其进行综合评价．体外弛豫结果表明，AI-EDA-DOTA-Gd的纵向弛豫效率（r₁=12.6mmol^-1·L·s^-1）是商用造影剂Gd-DOTA（r₁=5.8 mmol^-1·L·s^-1）的2.2倍．动物组织生理切片和溶血性实验结果说明其具有良好的生物相容性和较低的毒性．动物体内成像结果显示，AI-EDA-DOTA-Gd的最佳成像时间为30~70 min，注射AI-EDA-DOTA-Gd后的肝脏组织信号相比于未注射造影剂时增强了约（55.1±5.7）%．

关键词: 弛豫效率, 大分子配合物, 生物相容性, 氨基酸共聚物, MRI造影剂

Abstract: A macromolecular gadolinium complex was prepared as a magnetic resonance imaging (MRI) contrast agent. An ethlendiamine-modified aspartic acid-valine copolymer was synthesized, conjugated with DOTA, and then chelated with Gd³⁺(AI-EDA-DOTA-Gd). T₁-relaxivity of the agent (i.e., 12.6 mmol^-1·L·s^-1) was 2.2 times that of Gd-DOTA (i.e., 5.8 mmol^-1·L·s^-1). Hemolytic tests showed that the macromolecular contrast agent had good blood compatibility. Histological results demonstrated low toxicity of the agent in vivo. MRI experiments on rats demonstrated a prominent enhancement in liver after AI-EDA-DOTA-Gd injection, which persisted longer than that obtained by Gd-DOTA injection. The mean percentage enhancement in liver parenchyma was (55.1±5.7)% at 30~70 min after injection.

Key words: MRI contrast agent, relaxivity, macromolecule, biocompatibility, amino acid copolymer

中图分类号:

O482.53

湛游洋, 薛蓉, 祝云龙, 李晓晶, 裴奉奎, 冯江华. 氨基酸共聚物修饰的生物相容性MRI造影剂[J]. 波谱学杂志, 2016, 33(4): 635-645.

ZHAN You-yang, XUE Rong, ZHU Yun-long, LI Xiao-jing, PEI Feng-kui, FENG Jiang-hua. A Biocompatible Gadolinium-Based Amino Acid Copolymer Contrast Agent for Magnetic Resonance Imaging[J]. Chinese Journal of Magnetic Resonance, 2016, 33(4): 635-645.

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氨基酸共聚物修饰的生物相容性MRI造影剂

A Biocompatible Gadolinium-Based Amino Acid Copolymer Contrast Agent for Magnetic Resonance Imaging

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