波谱学杂志

• Varian公司专栏 • 上一篇    

高效液相核磁共振仪联用技术及微量探头技术在天然产物分析中的应用

王映红,贺文义,李小妹,李斌,刘欣,林茂   

  1. 中国医学科学院 中国协和医大药物所,北京 100050
  • 收稿日期:2002-07-25 出版日期:2002-09-05 发布日期:2002-09-05

APPLICATION OF LC-NMR AND NANO PROBE TECHNOLOGY IN NATURAL PRODUCTS

WANG Ying-hong, HE Wen-yi, LI Xiao-mei, LI Bing, LIU Xing, LIN Mao   

  1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Uni on Medical college, Beijing 100050, China
  • Received:2002-07-25 Online:2002-09-05 Published:2002-09-05

摘要:

以下工作在VARIAN公司的INOVA 500 MHz仪器上完成,使用了高灵敏度的LC-NMR联用探 头及先进的WET脉冲序列进行溶剂峰压制. 通过运用高效液相-核磁共振仪联用技术及微量探头技术对几个天然产物混合物进行分析,展示了高效液相-核磁共振联用(HPLC-NMR)技术及微量探头技术在天然产物分析中的优势,既高效,快速,微量.  二者结合使用,使得用NMR技术分析天然产物混合物变得切实可行.

关键词: 高效液相-核磁共振联用(HPLC-NMR)技术, 微量探头技术, 核磁共振谱

Abstract:

The technology of HPLC-NMR can act as an import action in the structure determi n ation of small amount mixtures difficult to separate. This technology can decrea se the difficulty in structure determination of the mixtures, improving the effi ciency, and worthy of being probed further during the work of separation and det ermination of small amount natural products. together with some other methods, s uch as Nano probe technology, relatively veracious ordinary NMR spectral excludi ng the disturbing impurity, the structures can be confirmed. this paper will giv e a description of this method that analyzes a few mixtures from natural product s.

Key words: Liquid chromatography-nuclear magnetic resonance, NMR spctroscopy, Nano probe, Ampelopsis sinica, Gnetum pendulum C.Y.Cheng, Groniothalamus Cheliensis

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